High prevalence of 2-mono- and 2,6-di-substituted Manol-terminating sequences among O-glycans released from brain glycopeptides by reductive alkaline hydrolysis
High prevalence of 2-mono- and 2,6-di-substituted Manol-terminating sequences among O-glycans released from brain glycopeptides by reductive alkaline hydrolysis
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DOI：
10.1046/j.1432-1327.1999.00572.x
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发表时间：
1999-08-01
期刊：
影响因子：
--
通讯作者：
Lawson, AM
中科院分区：
文献类型：
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作者：
Chai, WG;Yuen, CT;Lawson, AM
通过碱性硼氢化物处理释放后，从不可透析的总脑糖肽中分离出的二糖至七糖进行了质谱和核磁共振波谱分析，并辅以衍生新糖脂的TLC-MS分析。已经揭示了一个 Manol 末端寡糖家族，其中包括具有 2,6-二取代 Manol 的新序列：[图形]与之前描述的作为少数群体出现的 Manol 末端 HNK-1 抗原阳性链相反 [Yuen, C.-T, Chai, W., Loveless, R.W., Lawson, A.M., Margolis, R.U. & Feizi, T. (1997) J. Biol。陈省身。 272, 8924-8931]，上述寡糖含量丰富。这些化合物与经典的 N-乙酰氨基半乳糖醇封端寡糖的比例约为 1:3。因此，在高等真核生物中似乎存在与酵母型蛋白O-甘露糖基化相关的主要替代途径，其酶学基础和功能重要性现在需要研究。
Di- to heptasaccharides isolated from total nondialyzable brain glycopeptides after release by alkaline borohydride treatment have been subjected to mass spectrometric and nuclear magnetic resonance spectroscopic analyses supplemented by TLC-MS analyses of derived neoglycolipids. A family of Manol-terminating oligosaccharides has been revealed which includes novel sequences with a 2,6-disubstituted Manol:[GRAPHICS]In contrast to the Manol-terminating HNK-1 antigen-positive chains described previously that occur as a minor population [Yuen, C.-T, Chai, W., Loveless, R.W., Lawson, A.M., Margolis, R.U. & Feizi, T. (1997) J. Biol. Chern. 272, 8924-8931], the above oligosaccharides are abundant. The ratio of these compounds to the classical N-acetylgalactosaminitol-terminating oligosaccharides is about 1:3. Thus, there appears to be in higher eukaryotes a major alternative pathway related to the yeast-type protein O-mannosylation, the enzymatic basis and functional importance of which now require investigation.