Fluorescence lifetime imaging ophthalmoscopy in type 2 diabetic patients who have no signs of diabetic retinopathy
Fluorescence lifetime imaging ophthalmoscopy in type 2 diabetic patients who have no signs of diabetic retinopathy
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DOI：
10.1117/1.jbo.20.6.061106
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发表时间：
2015-06-01
影响因子：
3.5
通讯作者：
Dawczynski, Jens
中科院分区：
文献类型：
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作者：
Schweitzer, Dietrich;Deutsch, Lydia;Dawczynski, Jens
眼睛的时间分辨自身荧光用于检测没有糖尿病视网膜病变迹象的糖尿病患者的代谢改变。研究对象为37例2型糖尿病患者的1只眼和11例假性失明患者的1只眼，以及25例健康患者的1只眼和23例假性失明患者的1只眼。在对自身荧光衰减进行三指数拟合后，在两个光谱通道（490 < ch1 < 560 nm, 560 < ch2 < 700 nm）中，对相应组的寿命tau(i)、振幅alpha(i)和相对贡献Q(i)的直方图进行统计比较。通过使用Holm-Bonferroni方法和计算寿命和直方图的差异来估计单个荧光团的变化。ch1中tau(2)、tau(3)和alpha(3)直方图的中位数和平均值显示，食相糖尿病患者与年龄匹配的对照组之间差异最大（p < 0.000004）。与健康受试者相比，糖尿病患者ch1中tau(2)值接近360 ps的像素点的缺失，tau(2)值接近450 ps的像素点的增加，以及tau(3)值从相似的3000到3700 ps的变化表明游离黄素腺嘌呤二核苷酸的产生增加，晚期糖基化终产物（AGE）的积累，并且可能是眼底从游离到蛋白结合的还原烟酰胺腺嘌呤二核苷酸的变化。年龄也在晶状体中积累。(C)作者。由SPIE在知识共享署名3.0未移植许可下发布。
The time-resolved autofluorescence of the eye is used for the detection of metabolic alteration in diabetic patients who have no signs of diabetic retinopathy. One eye from 37 phakic and 11 pseudophakic patients with type 2 diabetes, and one eye from 25 phakic and 23 pseudophakic healthy subjects were included in the study. After a three-exponential fit of the decay of autofluorescence, histograms of lifetimes tau(i), amplitudes alpha(i), and relative contributions Q(i) were statistically compared between corresponding groups in two spectral channels (490 < ch1 < 560 nm, 560 < ch2 < 700 nm). The change in single fluorophores was estimated by applying the Holm-Bonferroni method and by calculating differences in the sum histograms of lifetimes. Median and mean of the histograms of tau(2), tau(3), and alpha(3) in ch1 show the greatest differences between phakic diabetic patients and age-matched controls (p < 0.000004). The lack of pixels with a tau(2) of similar to 360 ps, the increased number of pixels with tau(2) > 450 ps, and the shift of tau(3) from similar to 3000 to 3700 ps in ch1 of diabetic patients when compared with healthy subjects indicate an increased production of free flavin adenine dinucleotide, accumulation of advanced glycation end products (AGE), and, probably, a change from free to protein-bound reduced nicotinamide adenine dinucleotide at the fundus. AGE also accumulated in the crystalline lens. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.