Biofilm proteome: Homogeneity or versatility?
Biofilm proteome: Homogeneity or versatility?
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DOI：
10.1021/pr034044t
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发表时间：
2004-01-01
影响因子：
4.4
通讯作者：
Jouenne, T
中科院分区：
文献类型：
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作者：
Vilain, S;Cosette, P;Jouenne, T
与人类生物膜感染相关的问题是由生物膜的独特特征引起的，特别是其对抗生素的高水平耐药性。为解释这种抗菌药物耐药性而提出的假设之一是生物膜细胞的表型分化。尽管许多关于生物膜的研究强调了细菌附着到表面后的生理变化，但没有研究明确证明“生物膜”生理学。为了对这一主题辩论做出贡献，我们使用主成分分析来解释通过对浮游和无柄铜绿假单胞菌细胞的粗蛋白提取物进行二维凝胶电泳获得的电泳图上观察到的斑点数量变化。这些分析表明，附着的铜绿假单胞菌细胞的蛋白质组与其浮游对应物的蛋白质组不同。此外，我们发现无柄铜绿假单胞菌的蛋白质组强烈依赖于生物膜基质的性质。
The problems associated with biofilm infections in humans result from the distinct characteristics of biofilms, in particular their high level of resistance to antibiotics. One of the hypotheses that have been advanced to explain this resistance to antimicrobials is the phenotypic differentiation of biofilm cells. Although many studies on biofilms have highlighted physiological alterations following the attachment of bacteria to a surface, no studies have explicitly demonstrated a "biofilm" physiology. To contribute to this topical debate, we used principal component analysis to interpret spot quantity variations observed on electropherograms obtained by two-dimensional gel electrophoresis of crude protein extracts from planktonic and sessile Pseudomonas aeruginosa cells. These analyses showed that the proteome of attached P. aeruginosa cells differs from that of their planktonic counterparts. Furthermore, we found that the proteome of sessile P. aeruginosa is strongly dependent on the nature of the biofilm substratum.