Chromosome aberrations do not persist in the lymphocytes or bone marrow cells of mice irradiated in utero or soon after birth
Chromosome aberrations do not persist in the lymphocytes or bone marrow cells of mice irradiated in utero or soon after birth
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DOI：
10.1667/rr0718.1
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发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Nakamura, N.
中科院分区：
文献类型：
--
作者：
Nakano, M.;Kodama, Y.;Nakamura, N.
将不同年龄的小鼠暴露于1戈伊或2戈伊的X射线，并在动物20周龄时用染色体I和3的FISH染色测定外周血T细胞、脾细胞和骨髓细胞的易位频率。发现平均易位频率非常低（= 6周龄）。p53（Trp 53）依赖性细胞凋亡在消除异常细胞中的主要作用未被提出，因为与p53（-/-）母体（7.4%）的频率相比，辐射胎儿（无论p53基因状态如何）显示出较低的易位频率（p53（-/-）小鼠为1.8%，p53（+/-）小鼠为1.4%）。与此相反，各种类型的畸变被认为是在脾和肝细胞时，新生儿照射后不久进行检查，类似于在成人照射后的骨髓细胞中观察到的。我们解释的结果表明，胎儿细胞通常是敏感的诱导染色体畸变，但异常细胞不坚持，因为胎儿干细胞往往是免费的畸变和他们的后代取代预先存在的细胞群体在出生后的生长的动物。(C)2007年，辐射研究学会
Mice were exposed at various ages to 1 Gy or 2 Gy of X rays, and translocation frequencies in peripheral blood T cells, spleen cells, and bone marrow cells were determined with FISH painting of chromosomes I and 3 when the animals,were 20 weeks old. It was found that the mean translocation frequencies were very low (= 6 weeks old. A major role of p53 (Trp53)-dependent apoptosis for elimination of aberrant cells was not suggested because irradiated fetuses, regardless of the p53 gene status, showed low translocation frequencies (1.8% in p53(-/-) mice and 1.4% in p53(+/-) mice) compared to the frequency in the p53(-/-) mother (7.4%). In contrast, various types of aberrations were seen in spleen and liver cells when neonates were examined shortly after irradiation, similar to what was observed in bone marrow cells after irradiation in adults. We interpreted the results as indicating that fetal cells are generally sensitive to induction of chromosome aberrations but that the aberrant cells do not persist because fetal stem cells tend to be free of aberrations and their progeny replace the pre-existing cell populations during the postnatal growth of the animals. (C) 2007 by Radiation Research Society