Uptake and elimination of emerging polyfluoroalkyl substance F-53B in zebrafish larvae: Response of oxidative stress biomarkers
Uptake and elimination of emerging polyfluoroalkyl substance F-53B in zebrafish larvae: Response of oxidative stress biomarkers
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斑马鱼幼虫对新兴多氟烷基物质 F-53B 的吸收和消除：氧化应激生物标志物的反应
DOI：
10.1016/j.chemosphere.2018.10.025
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Wenqing Tu
中科院分区：
文献类型：
--
作者：
Yongming Wu;Mi Deng;Yuanxiang Jin;Xiyan Mu;Xiaoli He;Nha-Thi Luu;Chunyan Yang;Wenqing Tu
在中国的金属电镀行业中，6：2氯化聚氟醚磺酸盐(F-53B)被广泛用作全氟辛烷磺酸(PFOS)的灭雾剂已有几十年的历史。最近，在水环境和野生捕捞的鱼类中经常发现F-53B。然而，关于F-53B的摄取和消除动力学以及毒理学效应的研究很少。在本研究中，斑马鱼仔鱼(受精后72 h)暴露于F-53B(10,100μg/L)48 h，然后净化24 h，以检测F-53B在鱼类体内的积累和消除动态以及对无毒防御系统的反应。结果表明，F-53B在斑马鱼仔鱼体内的积累具有浓度和时间依赖性，BCF值为3 612~3 615，但消除较慢(半衰期为241.5~258.6 h)。F-53B暴露诱导斑马鱼幼体氧化应激，表现为GSH和MDA含量降低，CAT、SOD、CuZn-SOD和GSH-ST活性降低，GSH-Px活性和CAT、SOD蛋白水平升高。然而，这些氧化应激标志物在净化后除超氧化物歧化酶的蛋白水平下降外，其余均恢复到对照水平。总而言之，这项工作的结果表明，F-53B的行为类似全氟辛烷磺酸，在斑马鱼幼体中具有生物蓄积性和持久性，并进一步诱导氧化应激反应。
6:2 chlorinated polyfluorinated ether sulfonate (F-53B) has been widely applied as a mist suppressant to replace perfluorooctane sulfonate (PFOS) in the metal plating industry in China for decades. Recently, F-53B has been frequently identified in the aquatic environment and wild-caught fish. However, studies on the uptake and elimination kinetics, and the toxicological effects of F-53B were very scarce. In this study, zebrafish larvae (72 h post fertilization, hpf) were exposed to F-53B (10, 100μg/L) for 48 h, followed by a 24 h of depuration to examine both the dynamics of accumulation and elimination of F-53B and responses of antoxidant defense system in fish. The results showed that F-53B rapidly accumulated in zebrafish larvae in a concentration and time-dependent manner with BCF values of 3612–3615, but was eliminated slowly (half-life ranged from 241.5 to 258.6 h). F-53B exposure induced oxidative stress in zebrafish larvae, as reflected by the reduction in the GSH and MDA contents, CAT, SOD, CuZn-SOD, and GSH-ST activities, and the increase in GSH-Px activity as well as CAT and SOD protein levels. However, these oxidative stress markers were restored to control levels except for a decrease in protein level of SOD after depuration. Collectively, the results of this work indicate that F-53B behaves like PFOS and is bioaccumulative and persistent in zebrafish larvae, and further induced oxidative stress responses.