MACROPHAGE BIOMATERIAL INTERACTIONS - THE STIMULATION OF ENDOTHELIALIZATION
MACROPHAGE BIOMATERIAL INTERACTIONS - THE STIMULATION OF ENDOTHELIALIZATION
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DOI：
10.1067/mva.1989.vs0090588
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发表时间：
1989-04-01
影响因子：
4.3
通讯作者：
BURGESS, W
中科院分区：
文献类型：
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作者：
GREISLER, HP;DENNIS, JW;BURGESS, W
这项目前的研究分析了巨噬细胞/生物材料的相互作用作为内皮细胞增殖的调节器。收集兔腹膜巨噬细胞，接种(1)次。106cell/ml)，加入S改良的EAGLE培养液和10%低血小板血浆马血清的培养瓶中。巨噬细胞的形态特征、非特异性酯酶和细胞膜上的Fc(免疫球蛋白G)受体被鉴定。培养条件为(1)无假体材料，(2)涤纶，或(3)Polyglactin 910(PG910)(ethcon，Inc.，Somerville，N.J.)。这两种假体材料都被精细地粉碎在媒体上。培养5周后，巨噬细胞胞浆内可见PG910包涵体。胞浆内未见涤纶。从第5周至第10周每周收集三组条件培养液，离心，过滤，按系列稀释液加入培养的静止小鼠肺毛细血管内皮细胞。通过血清剥夺和[~3H]胸腺嘧啶核苷掺入实验证实细胞处于静止状态。在加入条件培养液16小时后，测量[~3H]胸腺嘧啶核苷，并以高于静止水平的百分比表示。从第6周到第10周，PG910组的有丝分裂活性逐渐增强。第10周，PG910组(1：10稀释)的DNA合成增加了620%。涤纶组与对照组(无假体)无差异。在7~10周内，[~3H]胸腺嘧啶核苷掺入平均增加了540%。65%；涤纶，323%。+-。65%，对照组343%。+-。26%(PG910与涤纶，p.ltoreq.0.004)。这些研究表明，巨噬细胞通过生物可吸收假体激活，产生生长因子释放，随后促进内皮细胞增殖。
This current study analyzed macrophage/biomaterial interactions as modulators of endothelial cell proliferation. Rabbit peritoneal macrophages were harvested and seeded (1 .times. 106 cells/ml) into culture flasks with Dulbecco''s modified Eagle medium and 10% platelet-poor, plasma-derived equine serum. Macrophages were identified by morphologic characteristics, nonspecific esterase, and Fc (immunoglobulin G) receptors on the cell membranes. Culture conditions were (1) no prosthetic material, (2) Dacron, or (3) Polyglactin 910 (PG910)(Ethicon, Inc., Somerville, N.J.). Both prosthetic materials were finely shredded into the media. After 5 weeks in culture, PG910 inclusions were seen within macrophage cytoplasm. No intracytoplasmic Dacron was observed. Conditioned media from all three groups were collected weekly from week 5 to week 10, centrifuged, filtered, and added in serial dilutions to cultured quiescent murine capillary lung endothelial cells. Quiescence was achieved by serum deprivation and verified by [3H] thymidine incorporation. Sixteen hours after addition of conditioned media, [3H] thymidine was measured in and expressed as percent increase above quiescent levels. Mitogenic activity in the PG910 group progressively increased from weeks 6 to 10. At week 10, the PG910 group (1:10 dilution) yielded a 620% increase in DNA synthesis. The Dacron group never varied from the control group (no prosthetic). The mean increases in [3H]thymidine incorporation over weeks 7 to 10 were PG910, 540% .+-. 65%; Dacron, 323% .+-. 65% and control, 343% .+-. 26% (PG910 vs Dacron, p .ltoreq. 0.004). These studies suggest macrophage activation by bioresorbable prostheses, yielding growth factor release with subsequent enhanced endothelial cell proliferation.