ID PROTEINS CONTROL GROWTH INDUCTION IN MAMMALIAN-CELLS
ID PROTEINS CONTROL GROWTH INDUCTION IN MAMMALIAN-CELLS
复制标题
DOI：
10.1073/pnas.91.11.4985
复制
发表时间：
1994-05-24
影响因子：
11.1
通讯作者：
PHILIPSON, L
中科院分区：
文献类型：
--
作者：
BARONE, MV;PEPPERKOK, R;PHILIPSON, L
Id 1、Id 2和Id 3（HLH 462）与碱性螺旋-环-螺旋蛋白家族的成员二聚化，但由于缺乏碱性区域，所得异二聚体不能结合DNA。因此，Id型蛋白质负调节碱性螺旋-环-螺旋蛋白质的DNA结合。在这里，我们报告说，Id 1，Id 2和Id 3诱导后不久，血清刺激逮捕NIH 3 T3。针对Id mRNA的反义寡核苷酸延迟了由血清或生长因子刺激引起的停滞细胞重新进入细胞周期。针对所有三种Id mRNA的反义寡核苷酸比单独的更有效。结合，这些结果表明，Id蛋白参与控制生长诱导。
Id1, Id2, and Id3 (HLH462) dimerize with members of the basic helix-loop-helix protein family, but due to the absence of the basic region, the resulting heterodimers cannot bind DNA. Therefore Id-type proteins negatively regulate DNA binding of the basic helix-loop-helix proteins. Here we report that Id1, Id2, and Id3 are induced shortly after serum stimulation in arrested NIH 3T3. Antisense oligonucleotides against the Id mRNAs delay the reentry of arrested cells into the cell cycle elicited by stimulation with serum or growth factors. Antisense oligonucleotides against all three Id mRNAs are more effective than individual ones. Combined, these results indicate that Id proteins are involved in the control of growth induction.