High-yield production of extracellular type-I cellulose by the cyanobacterium Synechococcus sp. PCC 7002.
High-yield production of extracellular type-I cellulose by the cyanobacterium Synechococcus sp. PCC 7002.
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DOI：
10.1038/celldisc.2015.4
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发表时间：
2015
期刊：
影响因子：
33.5
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Zhao C;Li Z;Li T;Zhang Y;Bryant DA;Zhao J
由cesA基因编码的纤维素合酶负责自然界中纤维素的合成。我们表明，细胞壁的蓝藻聚球藻7002天然含有纤维素。纤维素可能是内外膜之间的层压层，也是该蓝藻细胞外糖萼的重要组成部分。将木葡糖酸醋杆菌（Gluconacetobacter xylinus）的6个基因cmc-ccp-cesAB-cesC-cesD-bgl在聚球藻（Synechococcus sp. PCC 7002）中过表达，可获得高产的胞外I型纤维素。高水平的纤维素生产只发生在本地cesA基因失活时，细胞生长在低盐度。该系统提供了一种从阳光和CO2中生产无木质素纤维素的方法，用于生物燃料生产和其他生物技术应用。
Cellulose synthase, encoded by the cesA gene, is responsible for the synthesis of cellulose in nature. We show that the cell wall of the cyanobacterium Synechococcus sp. PCC 7002 naturally contains cellulose. Cellulose occurs as a possibly laminated layer between the inner and outer membrane, as well as being an important component of the extracellular glycocalyx in this cyanobacterium. Overexpression of six genes, cmc–ccp–cesAB–cesC–cesD–bgl, from Gluconacetobacter xylinus in Synechococcus sp. PCC 7002 resulted in very high-yield production of extracellular type-I cellulose. High-level cellulose production only occurred when the native cesA gene was inactivated and when cells were grown at low salinity. This system provides a method for the production of lignin-free cellulose from sunlight and CO2 for biofuel production and other biotechnological applications.