Embryonic interneurons from the medial, but not the caudal ganglionic eminence trigger ocular dominance plasticity in adult mice
Embryonic interneurons from the medial, but not the caudal ganglionic eminence trigger ocular dominance plasticity in adult mice
复制标题
DOI：
10.1007/s00429-016-1232-y
复制
发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Lehmann, Konrad
中科院分区：
文献类型：
--
作者：
Isstas, Marcel;Teichert, Manuel;Lehmann, Konrad
来自内侧神经节隆起（MGE）的含有细小蛋白的篮状细胞提供的皮层抑制成熟是在关键时期开始增强视觉皮层可塑性的关键事件。尽管人们普遍认为，进一步增加抑制会再次关闭临界期，但最近的研究表明，将来自MGE的胚胎中间神经元注入幼鼠的视觉皮层后，可以诱导额外的人工临界期。然而，这种效应是否确实是mge衍生的细胞所特有的，以及在完全成年的动物中是否也能诱导出关键时期样可塑性，目前还没有定论。为了澄清这些问题，我们将MGE或尾神经节隆起（CGE）的外植体注射到完全成年小鼠的视觉皮层，并在33天后进行4天的单眼剥夺。移植MGE细胞而非移植CGE细胞的动物表现出明显的眼优势可塑性。免疫组织化学证实，来自两种来源的注射细胞在宿主皮层中迁移很远，大多数发育成产生GABA的神经元，只有来自MGE的细胞表达小白蛋白。因此，我们的研究结果证实，胚胎中间神经元的可塑性诱导作用对来自MGE的细胞是特异性的，并且与宿主动物的年龄无关。
The maturation of cortical inhibition provided by parvalbumin-containing basket cells derived from the medial ganglionic eminence (MGE) is a key event in starting the enhanced visual cortical plasticity during the critical period. Although it is generally assumed that a further increase in inhibition closes the critical period again, it was recently shown that embryonic interneurons derived from the MGE can induce an additional, artificial critical period when injected into the visual cortex of young mice. It has, however, remained open whether this effect was indeed specific for MGE-derived cells, and whether critical period-like plasticity could also be induced in fully adult animals. To clarify these issues, we injected explants from either the MGE or the caudal ganglionic eminence (CGE) into the visual cortices of fully adult mice, and performed monocular deprivation 33 days later for 4 days. Animals implanted with MGE cells, but not with CGE cells, showed marked ocular dominance plasticity. Immunohistochemistry confirmed that the injected cells from both sources migrated far in the host cortex, that most developed into neurons producing GABA, and that only cells from the MGE expressed parvalbumin. Thus, our results confirm that the plasticity-inducing effect of embryonic interneurons is specific for cells from the MGE, and is independent of the host animal's age.