Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo
Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo
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DOI：
10.1038/ni1522
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发表时间：
2007-11-01
影响因子：
30.5
通讯作者：
Shortman, Ken
中科院分区：
文献类型：
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作者：
Naik, Shalin H.;Sathe, Priyanka;Shortman, Ken
树突状细胞(DC)功能特化亚型的发育可以通过细胞因子受体Flt3配体的骨髓培养来模拟。这种培养产生的DC类似于脾浆细胞样树突状细胞(PDCs)、CD8(+)常规DC(CDCs)和CD8(-)CDDC。在这里，我们从这样的培养中分离出两个顺序的DC-承诺前体细胞：分离前DC，这导致了过渡的前DC，在分化为三个不同的DC亚型(pDC，CD8(+)CDCs和CD8(-)CDCs)的过程中。我们还分离了体内等同于DC承诺的DC前体细胞，这也导致了三种DC亚型。对单个DC前体的克隆分析表明，一些DC前体产生了所有三种DC亚型，一些产生CDC而不产生pDC，一些完全致力于单一的DC亚型。因此，对特定DC亚型的承诺主要从这个亲DC阶段开始。
The development of functionally specialized subtypes of dendritic cells ( DCs) can be modeled through the culture of bone marrow with the ligand for the cytokine receptor Flt3. Such cultures produce DCs resembling spleen plasmacytoid DCs ( pDCs), CD8(+) conventional DCs (cDCs) and CD8(-) cDCs. Here we isolated two sequential DC-committed precursor cells from such cultures: dividing 'pro-DCs', which gave rise to transitional 'pre-DCs' en route to differentiating into the three distinct DC subtypes (pDCs, CD8(+) cDCs and CD8(-) cDCs). We also isolated an in vivo equivalent of the DC-committed pro-DC precursor cell, which also gave rise to the three DC subtypes. Clonal analysis of the progeny of individual pro-DC precursors demonstrated that some pro-DC precursors gave rise to all three DC subtypes, some produced cDCs but not pDCs, and some were fully committed to a single DC subtype. Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage.