Molecular Identity of Uncoupling Proteins in Thermogenic Skunk Cabbage
Molecular Identity of Uncoupling Proteins in Thermogenic Skunk Cabbage
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DOI：
10.1093/pcp/pcn161
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发表时间：
2008-12-01
影响因子：
4.9
通讯作者：
Inaba, Takehito
中科院分区：
文献类型：
--
作者：
Ito-Inaba, Yasuko;Hida, Yamato;Inaba, Takehito
据报道，产热臭鼬甘蓝具有两种类型的解偶联蛋白（UCP），典型的6-跨膜（TM）SrUCPA和非典型的5-TM SrUCPB。为了进一步验证SrUCPs在产热臭鼬菜中的作用，我们更详细地研究了SrUCPs的分子身份。mRNA和基因组分析均支持SrUCPA的存在，但不支持SrUCPB。此外，从肉穗花序线粒体中纯化的SrUCP蛋白经质谱鉴定为SrUCPA。这些结果清楚地表明，SrUCPA是臭鼬甘蓝中主要表达的UCP，并且非典型SrUCPB的存在不太可能与臭鼬甘蓝的产热有关。
Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.