Plasminogen activator activity is associated with neural crest cell motility in tissue culture.
Plasminogen activator activity is associated with neural crest cell motility in tissue culture.
复制标题
纤溶酶原激活剂活性与组织培养中的神经嵴细胞运动相关。
DOI：
10.1002/jez.1402510203
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Isseroff,RR
中科院分区：
文献类型：
--
作者：
Erickson,CA;Isseroff,RR
我们研究了诸如纤溶酶原激活物(PA)之类的蛋白酶对神经脊细胞非凡的运动能力做出贡献的可能性。我们发现，从体外分离的神经管中迁移出来的干神经脊细胞产生PA，并且在培养8天后细胞相关PA水平显著增加。这种增加不是分化或培养时间的结果，因为神经管顶部形成的神经脊细胞团分化成色素细胞，但不活动产生非常低水平的PA。如果这些簇从神经管中移除，并在它们迁移的塑料基质上复制，与细胞相关的PA水平显著增加，这表明PA的产生与运动性有关。PA/纤溶酶活性的抑制剂在体外显著降低神经脊细胞的运动能力，进一步支持了蛋白水解酶在神经脊细胞迁移中起重要作用的观点。
We have examined the possibility that proteases such as plasminogen activator (PA) contribute to the extraordinary motile capability of neural crest cells. We show that trunk neural crest cells that migrate from isolated neural tubes in vitro produce PA and that the level of cell‐associated PA increases dramatically after 8 days in culture. This increase is not the result of differentiation or time in culture, because neural crest cell clusters that form on top of the neural tube and differentiate into pigment cells but are immotile produce very low levels of PA. If these clusters are removed from the neural tube and replated on a plastic substratum where they migrate, the level of PA associated with the cells increases dramatically, suggesting that PA production is associated with motility. Inhibitors of PA/plasmin activity significantly reduce neural crest cell motility in vitro, further supporting the idea that proteases are important in neural crest cell migration.