Mechanism of initial reaction of phenylhydrazine with oxyhemoglobin and effect of ring substitutions on the biomolecular rate constant of this reaction.
Mechanism of initial reaction of phenylhydrazine with oxyhemoglobin and effect of ring substitutions on the biomolecular rate constant of this reaction.
复制标题
苯肼与氧合血红蛋白的初始反应机理以及环取代对该反应的生物分子速率常数的影响。
DOI：
10.1021/bi00539a022
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Matteson,JL
中科院分区：
文献类型：
--
作者：
Itano,HA;Matteson,JL
哈维Itano* 和Jeanne L.苯肼在氧气存在下引起血红蛋白氧化变性。这个过程的第一步是双分子反应，可能是从苯肼到氧合血红蛋白的双电子转移。这个反应的产物既不是高铁血红蛋白也不是脱氧血红蛋白。超氧化物歧化酶和过氧化氢酶消除了增加反应表观速率的副反应，在577 nm处用荧光光度法测量;羟基自由基和单线态氧的清除剂在有或没有这些酶的情况下都不影响该速率。卤素原子。向氧合血红蛋白溶液中加入苯肼，导致一种变性血红蛋白沉淀，其中血红素部分发生了不完全氧化变化。当完整红细胞发生氧化变性时，产生的细胞内沉淀物称为海因茨小体，其形成导致严重的溶血性贫血和网织红细胞增多症。虽然用苯肼诱导贫血是获得网织红细胞用于生化研究的最常用方法，但苯肼促进氧化变性的化学机制尚不清楚。芳肼诱发Heinz小体的严重程度
Harvey A. Itano* and Jeanne L. Matteson abstract: Phenylhydrazine in the presence of oxygen causes the oxidative denaturation of hemoglobin. The initial step in this process is a bimolecular reaction, probably a two-electron transfer from phenylhydrazine to oxyhemoglobin. The product of this reaction is neither methemoglobin nor deoxyhemoglobin. Superoxide dismutase and catalase eliminate side reactions that increase the apparent rate of this reaction as measured spectrophotometrically at577 nm; scavengers for the hydroxyl radical and singlet oxygen do notaffect this rate either in the presence or in theabsence of these enzymes. Halogen atoms. Addition of phenylhydrazine to a solution of oxyhemoglobin results in precipitation of a form of denatured hemoglobin in which the heme moiety has undergoneoxidative changes. When oxidative denaturation takes place in an intact erythrocyte, the resulting intracellular precipitates are known as Heinz bodies, formation of which leads to severe hemolytic anemia and reticulocytosis. Although induction of anemia with phenylhydrazine is the most common method for obtaining reticulocytes for biochemical studies, the chemical mechanism of phenylhydrazine-promoted oxidative denaturation is not known. The severity of arylhydrazine-induced Heinz-body