Uncoupling PR gene expression from NPR1 and bacterial resistance:: Characterization of the dominant Arabidopsis cpr6-1 mutant
Uncoupling PR gene expression from NPR1 and bacterial resistance:: Characterization of the dominant Arabidopsis cpr6-1 mutant
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DOI：
10.1105/tpc.10.4.557
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发表时间：
1998-04-01
期刊：
影响因子：
11.6
通讯作者：
Dong, XN
中科院分区：
文献类型：
--
作者：
Clarke, JD;Liu, YD;Dong, XN
在拟南芥中，NPR1 介导水杨酸 (SA) 诱导的发病相关 (PR) 基因和系统获得性抗性 (SAR) 的表达。在这里，我们报告了另一个成分 CPR6 的鉴定，它可能与 NPR1 一起调节 PR 基因表达。显性 CPR6-1 突变体表达 SA/NPR1 调节的 PR 基因（PR-1、BGL2 和 PR-5），并且在没有 SAR 诱导的情况下，对丁香假单胞菌 pv maculicola ES4326 和 Peronospora parasitica Noco2 表现出增强的抗性。 cpr6-1 诱导的 PR 基因表达在 cpr6-1 npr1-1 双突变体中不受抑制，但当 SA 被水杨酸羟化酶去除时受到抑制。因此，cpr6-1 中 PR 基因的组成型表达需要 SA 而不是 NPR1。此外，对 P. s 的抵抗力。尽管表达了 PR-1、BGL2 和 PR-5，maculicola ES4326 在 cpr6-1 npr1-1 双突变体中受到抑制，因此，对 P.s、maculicola ES4326 的抗性必须通过受 NPR1 调节的未鉴定抗菌基因产物来实现。这些结果表明CPR6是参与植物防御的多种信号转导途径的重要调节因子。
In Arabidopsis, NPR1 mediates the salicylic acid (SA)-induced expression of pathogenesis-related (PR) genes and systemic acquired resistance (SAR), Here, we report the identification of another component, CPR6, that may function with NPR1 in regulating PR gene expression. The dominant CPR6-1 mutant expresses the SA/NPR1-regulated PR genes (PR-1, BGL2, and PR-5) and displays enhanced resistance to Pseudomonas syringae pv maculicola ES4326 and Peronospora parasitica Noco2 in the absence of SAR induction. cpr6-1-induced PR gene expression is not suppressed in the cpr6-1 npr1-1 double mutant but is suppressed when SA is removed by salicylate hydroxylase. Thus, constitutive PR gene expression in cpr6-1 requires SA but not NPR1. In addition, resistance to P. s. maculicola ES4326 is suppressed in the cpr6-1 npr1-1 double mutant, despite expression of PR-1, BGL2, and PR-5, Resistance to P. s, maculicola ES4326 must therefore be accomplished through unidentified antibacterial gene products that are regulated through NPR1. These results show that CPR6 is an important regulator of multiple signal transduction pathways involved in plant defense.