The effects of hyaluronan during the development of osteoarthritis
The effects of hyaluronan during the development of osteoarthritis
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DOI：
10.1016/s1063-4584(97)80021-0
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发表时间：
1997-07-01
影响因子：
7
通讯作者：
Amiel, D
中科院分区：
文献类型：
--
作者：
Yoshioka, M;Shimizu, C;Amiel, D
将99只成年新西兰白色（NZW）兔行单侧前交叉韧带横断（ACLT），分为3组。对侧非手术膝关节作为对照。第一组（SA）在ACLT后4周开始接受0.3 ml透明质酸（HA：MW; 8x 10（5））关节内注射，每周一次，持续5周。第二组（SV）以与SA组相同的方式注射溶剂（HA载体）。第三组（SN）为ACLT后非治疗组。术后9周处死所有动物，并通过大体形态学、组织形态计量学和生化分析进行评估。SA组股骨软骨的大体形态学变化较SV组和SN组轻。测量了软骨厚度、软骨面积和滑膜衬里细胞层厚度的组织形态计量学参数，表明HA对关节软骨和滑膜组织的保存具有积极作用。类似地，如通过生物化学分析所测量的，来自注射HA的膝关节的软骨和滑膜组织与对侧对照相比没有表现出显著的改变[即，水含量、吡啶啉浓度、软骨的糖胺聚糖（GAG）含量和滑膜组织的DNA浓度]。
Ninety-nine mature New Zealand White (NZW) rabbits underwent unilateral anterior cruciate ligament transection (ACLT) and were divided into three groups. The contralateral non-operated knees served as controls. The first group (SA) received intra-articular injections of 0.3 ml hyaluronan (HA: MW; 8x10(5)) beginning 4 weeks after ACLT, once a week for 5 weeks. The second group (SV) was injected with vehicle (carrier of HA) in the same fashion as the SA group. The third group (SN) served as a nontreatment group post ACLT. All animals were killed 9 weeks post-surgery and were assessed by gross morphology, histomorphometry and biochemical analysis. Gross morphologic changes on the femoral cartilage in the SA group were less severe than those in the SV and SN groups. Cartilage thickness, cartilage area, and thickness of synovial lining cell layer histomorphometric parameters were measured, showing a positive effect of HA on the preservation of articular cartilage and synovial tissue. Similarly, the cartilage and synovial tissues from knees injected with HA did not demonstrate significant alterations from contralateral controls as measured by biochemical analysis [i.e., water content, pyridinoline concentration, glycosaminoglycan (GAG) content for the cartilage, and DNA concentration for the synovial tissue].