Modification of GABAB1 and GABAB2 receptor subunits in the somatosensory cerebral cortex and thalamus of rats with absence seizures (GAERS)
Modification of GABAB1 and GABAB2 receptor subunits in the somatosensory cerebral cortex and thalamus of rats with absence seizures (GAERS)
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DOI：
10.1016/s0920-1211(03)00090-1
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发表时间：
2003-06-01
影响因子：
2.2
通讯作者：
Bowery, NG
中科院分区：
文献类型：
--
作者：
Princivalle, AP;Richards, DA;Bowery, NG
在本研究中，我们研究了来自斯特拉斯堡的遗传性失神癫痫大鼠（GAERS）的躯体感觉皮层（SI）和丘脑腹基底核（VB）和网状核（Rt）中GABA（B）受体的表达，这是人类失神癫痫的动物模型。我们把注意力集中在丘脑皮层网络上，因为已经证明，失神发作是在这个特定的回路中产生的，它是在几个抑制性系统（如GABA）和兴奋性系统的控制下。用GABA（B）受体拮抗剂\H-3\CGP 62349获得的放射自显影数据显示，对照大鼠和GAERS之间的Kd或Bmax值没有任何差异。原位杂交（ISH）结果显示，GABA（B1）的信使RNA在SI和VB丘脑核，但不是在R1丘脑核的减少显着增加。相反，免疫细胞化学数据显示，GABA（B1）和GABA（B2）受体亚单位的表达增加，在所有的区域检查。躯体感觉大脑皮层与对照组相比，GAERS组丘脑VB和Rt核的分布与对照组相符合，主要发现是GAERS组皮质丘脑回路中GABA（B）受体蛋白表达上调。(C)2003 Elsevier B. V.保留所有权利。
In the present study, we have investigated GABA(B) receptor expression in somatosensory cortex (SI) and the ventrobasal (VB) and reticular (Rt) thalamic nuclei of Genetic Absence Epilepsy Rats from Strasbourg (GAERS), which represent an animal model for the human absence epilepsy. We focused our attention oil the thalamocortical network because it has been demonstrated that absence seizures are generated in this specific circuit, which is under the control of several inhibitory, e.g. GABA, and excitatory systems.Autoradiography data obtained with the GABA(B) receptor antagonist \H-3\CGP62349 did not show any differences in K-d or B-max values between control rats and GAERS. In situ hybridisation (ISH) results showed a significant increase in messenger RNA for GABA(B1) in the SI and a decrease in the VB thalamic nucleus but not in the R1 thalamic nucleus. By contrast the immunocytochemical data revealed an increased expression of both GABA(B1) and GABA(B2) receptor subunits in all the regions examined. somatosensory cerebral cortex. VB thalamus and Rt nucleus fit GAERS compared to controls.The main finding was an up-regulation of GABA(B) receptor protein in the corticothalmic circuit in GAERS compared to Controls. (C) 2003 Elsevier B.V. All rights reserved.