Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance
Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance
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DOI：
10.1094/pdis-08-21-1599-re
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发表时间：
2022-03-28
期刊：
影响因子：
4.5
通讯作者：
Kang, Houyang
中科院分区：
文献类型：
--
作者：
Gong, Biran;Zhang, Hao;Kang, Houyang
由条锈菌引起的条锈病。白粉菌引起的小麦白粉病和白粉病。小麦黑粉病是世界范围内危害小麦的毁灭性病害。从栽培小麦和野生近缘植物中探索新的抗病基因是减少用于抗击这些疾病的杀菌剂数量的最有效手段。黑麦草(Thinopyrum sCirpeum，2n=4x=28，eeee)是小麦抗条锈病和抗白粉病等有用基因的重要储存库，可用于小麦抗病育种。在这里，我们描述了一种新的小麦-Th。建立了苦参二体代换系K16-730-3和可用于Th示踪的染色体特异标记。染色体或染色体片段转移到小麦中。基因组原位杂交和荧光原位杂交分析表明，K16-730-3是一个新的4E(4D)染色体代换系。苗期和成株期的病害反应评价表明，K16-730-3对条锈病和白粉病具有较强的抗性。此外，K16-730-3与其亲本在产量上没有明显差异。测序分型分析表明，74个基于PCR的标记可以准确地追踪到4E染色体，这些标记与小麦背景中的抗病基因连锁。进一步的标记验证分析表明，13个特异标记可以区分Th的E基因组染色体。和其他小麦近缘种的染色体。携带条锈病和白粉病抗性基因的新代换系K16-730-3可作为抗病育种的新种质。本研究开发的分子标记可用于提高小麦抗病性的分子标记辅助选择。
Stripe rust caused by Puccinia striiformis f. sp. tritici and powdery mildew caused by Blumeria graminis f. sp. tritici are devastating diseases of wheat worldwide. Exploration of new disease-resistant genes from cultivated wheat and wild relatives are the most effective means of reducing the amounts of fungicides applied to combat these diseases. Thinopyrum scirpeum (2n = 4x = 28, EEEE) is an important promising reservoir of useful genes, including stripe rust and powdery mildew resistance, and may be useful for increasing wheat disease resistance. Here, we characterize a novel wheat-Th. scirpeum disomic substitution line, K16-730-3, and chromosome-specific markers were developed that can be used to trace the Th. scirpeum chromosome or chromosome segments transferred into wheat. Genomic in situ hybridization and fluorescence in situ hybridization analyses indicated that K16-730-3 is a new 4E (4D) chromosomal substitution line. Evaluation of seedling and adult disease responses revealed that K16-730-3 is resistant to stripe rust and powdery mildew. In addition, no obvious difference in grain yield was observed between K16-730-3 and its wheat parents. Genotyping-by-sequencing analyses indicated that 74 PCR-based markers can accurately trace chromosome 4E, which were linked to the disease resistance genes in the wheat background. Further marker validation analyses revealed that 13 specific markers can distinguish between the E-genome chromosomes of Th. scirpeum and the chromosomes of other wheat-related species. The new substitution line K16-730-3 carrying the stripe rust and powdery mildew resistance genes will be useful as novel germplasm in breeding for disease resistance. The markers developed in this study can be used in marker-assisted selection for increasing disease resistance in wheat.