Blunt-ended Cloning into Plasmid Vectors.

Blunt-ended Cloning into Plasmid Vectors.
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DOI:
10.1101/pdb.prot3921
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发表时间:
2006-06
期刊:
CSH protocols
影响因子:
--
通讯作者:
J. Sambrook;David W. Russell
J. Sambrook;David W. Russell
中科院分区:
其他
文献类型:
--
作者:
J. Sambrook;David W. Russell

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方法1.在单独的反应中,用产生平末端的适当限制性内切酶消化1-10 μg质粒DNA和外源DNA。
METHOD1. In separate reactions, digest 1-10 μg of the plasmid DNA and foreign DNA with the appropriate restriction enzyme (s) that generate blunt ends.