A guide to simple and informative binding assays.
A guide to simple and informative binding assays.
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DOI：
10.1091/mbc.e10-08-0683
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发表时间：
2010-12
影响因子：
3.3
通讯作者：
Pollard TD
中科院分区：
文献类型：
--
作者：
Pollard TD
结合测定的目的是测量两种分子之间的相互作用，例如蛋白质结合另一种蛋白质、小分子或核酸。制备试剂需要艰苦的工作，但许多结合实验的设计缺陷限制了所获得的信息。特别是许多实验不能测量反应物之间的亲和力。本文介绍了在结合实验中充分利用有价值的试剂的简单方法。
The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments.