Same Locus for Non-shattering Seed Pod in Two Independently Domesticated Legumes, Vigna angularis and Vigna unguiculata
Same Locus for Non-shattering Seed Pod in Two Independently Domesticated Legumes, Vigna angularis and Vigna unguiculata
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DOI：
10.3389/fgene.2020.00748
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发表时间：
2020-07
影响因子：
3.7
通讯作者：
Yu Takahashi;Alisa Kongjaimun;Chiaki Muto;Y. Kobayashi;M. Kumagai;H. Sakai;K. Satou;Kuniko Teruya;Akino Shiroma;Makiko Shimoji;Takashi Hirano;T. Isemura;Hiroki Saito;A. Baba-Kasai;A. Kaga;P. Somta;N. Tomooka;K. Naito
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文献类型：
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作者：
Yu Takahashi;Alisa Kongjaimun;Chiaki Muto;Y. Kobayashi;M. Kumagai;H. Sakai;K. Satou;Kuniko Teruya;Akino Shiroma;Makiko Shimoji;Takashi Hirano;T. Isemura;Hiroki Saito;A. Baba-Kasai;A. Kaga;P. Somta;N. Tomooka;K. Naito
豆荚破碎性的丧失是豆科作物最重要的驯化相关性状之一。不破碎的表型要么是通过扰动瓣膜之间离层的形成，要么是通过内果皮厚壁组织中螺旋张力的丧失来实现，从而使豆荚裂开以分散种子。在驯化过程中，红豆 (Vigna angularis) 和长豆 (Vigna unguiculata cv-gr. Sesquipedalis) 的厚壁组织减少或消失，从而导致种荚的破碎行为。在这里，我们对回交群体进行了精细作图，并将小豆中的候选基因组区域缩小至 4 kbp，将长豆中的候选基因组区域缩小至 13 kbp。在位于这些区域的基因中，我们在小豆、长豆甚至豇豆中发现了编码截短蛋白的 MYB26 基因。因此，我们的研究结果表明，两种豆科植物的独立驯化选择了具有相同性状的相同基因座。我们还认为，MYB26 可能是改善其他豆类（例如大豆）破碎表型的靶基因。
Loss of pod shattering is one of the most important domestication-related traits in legume crops. The non-shattering phenotypes have been achieved either by disturbed formation of abscission layer between the valves, or by loss of helical tension in sclerenchyma of endocarp, that split open the pods to disperse the seeds. During domestication, azuki bean (Vigna angularis) and yard-long bean (Vigna unguiculata cv-gr. Sesquipedalis) have reduced or lost the sclerenchyma and thus the shattering behavior of seed pods. Here we performed fine-mapping with backcrossed populations and narrowed the candidate genomic region down to 4 kbp in azuki bean and 13 kbp in yard-long bean. Among the genes located in these regions, we found MYB26 genes encoded truncated proteins in azuki bean, yard-long bean, and even cowpea. As such, our findings indicate that independent domestication on the two legumes has selected the same locus for the same traits. We also argue that MYB26 could be a target gene for improving shattering phenotype in other legumes, such as soybean.