Novel Putative Bluetongue Virus Serotype 29 Isolated from Inapparently Infected Goat in Xinjiang of China
Novel Putative Bluetongue Virus Serotype 29 Isolated from Inapparently Infected Goat in Xinjiang of China
复制标题
从中国新疆隐性感染的山羊中分离出新型推定蓝舌病毒血清型 29
DOI：
10.1111/tbed.13927
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发表时间：
2021
影响因子：
4.3
通讯作者：
Huachun Li
中科院分区：
文献类型：
--
作者：
Heng Yang;Wenxi Gu;Zhanhong Li;Ling Zhang;Defang Liao;Jianling Song;Baoxin Shi;Jiapaer Hasimu;Zhuoran Li;Zhenxing Yang;Qi Zhong;Huachun Li
蓝舌病病毒（BTV）是引起羊、牛等反刍动物蓝舌病的环状病毒属的模式种。在全球范围内确定了24种BTV血清型和几种非典型血清型。本研究从新疆尉犁县1只无症状哨羊中分离到1株BTV（V196/XJ/2014）。通过血清型特异性常规RT-PCR和BTV-1至BTV-27的真实的实时RT-PCR，以及使用BTV-1至BTV-24参考血清的病毒中和试验，该分离株的血清型鉴定显示一致的阴性结果。基因组分析显示V196/XJ/2014与BTV-25至BTV-28、BTV-X/XJ 1407、BTV-X/ITL 2015和BTV-Y/TU 2017的非典型血清型分组，而V196/XJ/2014的片段2和VP 2蛋白分别为<63.4%/61.4%在系统发育树中，BTV的核酸和氨基酸序列与其它血清型一致，其片段2形成了一个独立的“核型”。这些结果表明V196/XJ/2014不属于任何已报道的BTV血清型。进一步的感染性和致病性研究表明，感染V196/XJ/2014的山羊没有表现出观察到的临床症状，但感染后检测到高水平的病毒扩增和同源中和抗体。我们的研究表明，BTV-29在中国新疆分离到一种新的推定血清型，这扩大了我们对BTV多样性的认识。
Bluetongue virus (BTV) is the ‘type’ species of the genusOrbiviruscausing bluetongue (BT) in sheep, bovine and other ruminants. Twenty‐four serotypes and several atypical serotypes of BTV were identified worldwide. In present study, a novel strain of BTV (V196/XJ/2014) was isolated from an asymptomatic sentinel goat in Yuli County, Xinjiang of China. Serotype identification of this isolate exhibited uniform negative results by serotype‐specific conventional RT‐PCR and real‐time RT‐PCR for BTV‐1 to BTV‐27, and virus neutralization tests using reference sera of BTV‐1 to BTV‐24. Genomic analysis showed V196/XJ/2014 grouped with atypical serotypes of BTV‐25 to BTV‐28, BTV‐X/XJ1407, BTV‐X/ITL2015 and BTV‐Y/TUN2017, while segment 2 and VP2 protein of V196/XJ/2014 shared <63.4%/61.4% nucleic acids and amino acids sequence identities with other recognized BTV serotypes and its segment 2 formed a separate ‘nucleotype’ in phylogenetic tree. These results indicated V196/XJ/2014 does not belong to any reported serotypes of BTV. Further studies of infectivity and pathogenicity showed that goats infected with V196/XJ/2014 did not exhibit observed clinical symptoms, but high level of virus amplification and homologous neutralization antibodies were detected post‐infection. Our studies suggested a novel putative serotype of BTV‐29 was isolated in Xinjiang of China, which expands our knowledge about the diversity of BTV.