Establishment of vagal sensorimotor circuits during fetal development in rats.
Establishment of vagal sensorimotor circuits during fetal development in rats.
复制标题
大鼠胎儿发育过程中迷走神经感觉运动回路的建立。
DOI：
10.1002/neu.480240509
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Levitt,P
中科院分区：
文献类型：
--
作者：
Rinaman,L;Levitt,P
研究了胎儿大鼠迷走神经运动神经元的分化及其与迷走神经感觉传入神经的中枢关系。为了识别周围突出的感觉和运动神经元，在固定制剂中，将1,1 ' -二十八烷基3,3,3 ',3 ' -四甲基多碳氰过氯酸盐（DiI）插入近端肠或颈迷走神经。在胚胎日(E) 12，标记的迷走感觉神经元存在于结节神经节中，少数感觉轴突投射到髓质背外侧。中枢感觉过程在E13和E14之间变得越来越普遍，但仍然局限于孤立束。迷走神经运动神经元首先在E13处被标记，聚集在与歧义核（NA）对应的区域内。额外的运动神经元似乎从第四脑室的生发区向NA迁移。迷走神经背侧运动核（DMV）中的运动神经元首先在E14处投射到肠道，并通过E16与心室区保持物理接触。感觉轴突在E15从孤立束出现，并通过孤立束核（NST）区域向内侧投射，最终到达心室区。当迷走神经感觉纤维在DMV和DMV树突延伸到NST内时，迷走神经感觉运动直接相互作用的可能底物出现在E16。到E18，迷走神经核明显成熟。这些数据表明，在胎儿大鼠中，迷走神经感觉传入和运动神经元树突的特异性和离散靶向性，并确定了在迷走神经感觉运动回路建立之前的有序发育事件序列。©1993 John Wiley & Sons, Inc
The differentiation of vagal motor neurons and their emerging central relationship with vagal sensory afferents was examined in fetal rats. To identify peripherally projecting sensory and motor neurons, 1,1′‐dioctadecyl 3,3,3′,3′‐tetramethylindocarbocyanine perchloarate (DiI) was inserted into the proximal gut or cervical vagus nerve in fixed preparations. At embryonic day (E) 12, labeled vagal sensory neurons are present in the nodose ganglia and a few sensory axons project into the dorsolateral medulla. Central sensory processes become increasingly prevalent between E13 and E14 but remain restricted to the solitary tract. Vagal motor neurons are first labeled at E13, clustered within a region corresponding to the nucleus ambiguus (NA). Additional motor neurons appear to be migrating toward the NA from the germinal zone of the fourth ventricle. Motor neurons in the dorsal motor nucleus of the vagus (DMV) first project to the gut at E14 and have processes that remain in physical contact with the ventricular zone through E16. Sensory axons emerge from the solitary tract at E15 and project medially through the region of the nucleus of the solitary tract (NST) to end in the ventricular zone. A possible substrate for direct vagovagal, sensorimotor interaction appears at E16, when vagal sensory fibers arborize within the DMV and DMV dendrites extend into the NST. By E18, the vagal nuclei appear remarkably mature. These data suggest specific and discrete targeting of vagal sensory afferents and motor neuron dendrites in fetal rats and define an orderly sequence of developmental events that precedes the establishment of vagal sensorimotor circuits. © 1993 John Wiley & Sons, Inc.