Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis
Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis
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DOI：
10.1039/b712330b
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ino, Kosuke;Okochi, Mina;Honda, Hiroyuki
为了了解单个细胞的行为，单细胞分析引起了人们的关注，因为大多数基于细胞的测定提供的数据具有大量细胞的平均值。单细胞的操作和分析技术对于理解单个细胞的行为至关重要。在本研究中，我们利用磁力和针座开发了单细胞培养阵列，可以将磁性标记的细胞分配在阵列上，并分析了它们的动态。销瓶座由磁性软铁制成，表面有 6000 多个柱子。将销钉固定器放置在磁铁上，以将磁通密度集中在柱子上方。将用磁铁矿阳离子脂质体 (MCL) 标记的 NIH/3T3 成纤维细胞接种到培养皿中，并将培养皿放置在带有磁铁的针座上。磁性标记的细胞在柱子所在的表面上被引导，并以高分辨率分配在阵列上。通过调整接种的细胞数量实现单细胞图案化，并在用磁铁移除针座后通过显微操作器收集目标细胞。此外，通过显微镜观察分析磁性图案细胞的形态变化，并观察细胞随时间的推移在阵列上的铺展。细胞培养阵列上基于磁力的细胞图案将是一种适合在细胞间变异和细胞间相互作用研究中分析细胞行为的技术。
In order to understand the behavior of individual cells, single cell analyses have attracted attention since most cell-based assays provide data with values averaged across a large number of cells. Techniques for the manipulation and analysis of single cells are crucial for understanding the behavior of individual cells. In the present study, we have developed single cell culture arrays using magnetic force and a pin holder, which enables the allocation of the magnetically labeled cells on arrays, and have analyzed their dynamics. The pin holder was made from magnetic soft iron and contained more than 6000 pillars on its surface. The pin holder was placed on a magnet to concentrate the magnetic flux density above the pillars. NIH/3T3 fibroblasts that were labeled with magnetite cationic liposomes (MCLs) were seeded into a culture dish, and the dish was placed over the pin holder with the magnet. The magnetically labeled cells were guided on the surface where the pillars were positioned and allocated on the arrays with a high resolution. Single-cell patterning was achieved by adjusting the number of cells seeded, and the target cell was collected by a micromanipulator after removing the pin holder with the magnet. Furthermore, change in the morphology of magnetically patterned cells was analyzed by microscopic observation, and cell spreading on the array was observed with time duration. Magnetic force-based cell patterning on cell culture arrays would be a suitable technique for the analysis of cell behavior in studies of cell-cell variation and cell-cell interactions.