[125I]fibrin deposition occurs at both early and late intervals of IgE-dependent or contact sensitivity reactions elicited in mouse skin. Mast cell-dependent augmentation of fibrin deposition at early intervals in combined IgE-dependent and contact sensitivity reactions.
[125I]fibrin deposition occurs at both early and late intervals of IgE-dependent or contact sensitivity reactions elicited in mouse skin. Mast cell-dependent augmentation of fibrin deposition at early intervals in combined IgE-dependent and contact sensitivity reactions.
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[125I]纤维蛋白沉积发生在小鼠皮肤中引起的IgE依赖性或接触敏感性反应的早期和晚期。
DOI：
10.4049/jimmunol.145.11.3719
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发表时间：
1990
影响因子：
4.4
通讯作者：
S. Galli
中科院分区：
文献类型：
--
作者：
Y. Mekori;S. Galli
当在小鼠皮肤中引发时，IgE依赖性速发型超敏反应或T细胞依赖性接触敏感性（CS）反应导致[125 I]纤维蛋白原局部外渗和[125 I]纤维蛋白沉积。然而，这两种类型的反应在动力学和对IgE、肥大细胞或T细胞的需求方面不同。在本研究中，我们研究了[125 I]纤维蛋白沉积的动力学和幅度在结合IgE依赖性和CS反应引起的同时在同一网站和比较的结果时，这两个反应引起在不同的网站。我们发现，[125 I]纤维蛋白沉积在纯IgE依赖性反应是更大的挑战后2或6小时比在24小时，但显着的纤维蛋白沉积持续在这些网站24小时后的挑战。在CS反应中，早在激发后2小时就检测到[125 I]纤维蛋白沉积，表明纤维蛋白沉积伴随着货车Lovern等人使用组织肿胀测量检测到的CS的“早期成分”。但在Ag激发后24 h，CS反应中的[125I]纤维蛋白沉积比2或6 h时多得多。当IgE依赖性和CS反应引起在同一网站，[125I]纤维蛋白沉积在早期间隔（2至6小时）后的挑战增加了3至25倍，在孤立的CS反应相比，但在24小时的结果在合并的反应几乎是相同的CS反应。在遗传性肥大细胞缺陷和同类正常小鼠中的研究表明，肥大细胞是IgE依赖性增强[125 I]纤维蛋白沉积的表达所必需的，在IgE依赖性和CS反应的早期间隔观察到，但不是与“纯”CS反应相关的[125 I]纤维蛋白沉积。这些研究结果表明，CS反应的IgE依赖性肥大细胞活化的净效应是增加与这些反应相关的纤维蛋白沉积，但这种效应仅在反应激发后的早期间隔才被认识到。
When elicited in the skin of mice, either IgE-dependent immediate hypersensitivity reactions or T cell-dependent contact sensitivity (CS) reactions result in local extravasation of [125I]fibrinogen and deposition of [125I]fibrin. However, these two types of reaction differ in kinetics and in requirement for IgE, mast cells, or T cells. In the present study, we investigated the kinetics and magnitude of [125I]fibrin deposition in combined IgE-dependent and CS reactions elicited simultaneously at the same site and compared the results with those obtained when the two reactions were elicited at separate sites. We found that [125I]fibrin deposition in pure IgE-dependent reactions was greater at 2 or 6 h after challenge than at 24 h, but that significant fibrin deposition persisted at those sites 24 h after challenge. In CS reactions, [125I]fibrin deposition was detected as early as 2 h after challenge, indicating that fibrin deposition accompanies the "early component" of CS detected by Van Loveren et al. with the use of measurements of tissue swelling. But much more [125I]fibrin deposition was present in CS reactions at 24 h than at 2 or 6 h after Ag challenge. When IgE-dependent and CS reactions were elicited at the same site, [125I]fibrin deposition at early intervals (2 to 6 h) after challenge was increased three- to 25-fold compared with that seen in isolated CS reactions, but at 24 h the results in the combined reactions were virtually identical to those in CS responses. Studies in genetically mast cell-deficient and congenic normal mice indicated that mast cells were required for expression of the IgE-dependent augmentation of [125I]fibrin deposition observed at early intervals in combined IgE-dependent and CS reactions, but not for the [125I]fibrin deposition associated with "pure" CS reactions. These findings indicate that the net effect of IgE-dependent mast cell activation on CS responses is to increase the fibrin deposition associated with these responses, but this effect is appreciated only at early intervals after elicitation of the reaction.