OSA: a fast and accurate alignment tool for RNA-Seq
OSA: a fast and accurate alignment tool for RNA-Seq
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DOI：
10.1093/bioinformatics/bts294
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发表时间：
2012-07-15
期刊：
影响因子：
5.8
通讯作者：
Liu, Kejun
中科院分区：
文献类型：
--
作者：
Hu, Jun;Ge, Huanying;Liu, Kejun
将 RNA-Seq 读数准确映射到参考基因组是执行转录本组装、异构体检测和定量等下游分析的关键步骤。许多工具已经被开发出来；然而，考虑到下一代测序数据集的巨大规模和转录组的复杂性，随着数据量的不断增加，RNA-Seq读图映射仍然是一个挑战。我们开发了 Omicsoft 序列比对器 (OSA)，这是一种快速、准确的 RNA-Seq 数据比对工具。以现有方法为基准，OSA 将绘图速度提高了 4-10 倍，灵敏度更高，误报率更低。
Accurately mapping RNA-Seq reads to the reference genome is a critical step for performing downstream analysis such as transcript assembly, isoform detection and quantification. Many tools have been developed; however, given the huge size of the next generation sequencing datasets and the complexity of the transcriptome, RNA-Seq read mapping remains a challenge with the ever-increasing amount of data. We develop Omicsoft sequence aligner (OSA), a fast and accurate alignment tool for RNA-Seq data. Benchmarked with existing methods, OSA improves mapping speed 4-10-fold with better sensitivity and less false positives.