A new promoter for α1C subunit of human L-type cardiac calcium channel CaV1.2
A new promoter for α1C subunit of human L-type cardiac calcium channel CaV1.2
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DOI：
10.1016/s0006-291x(02)00894-x
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发表时间：
2002-08-16
影响因子：
3.1
通讯作者：
Palade, P
中科院分区：
文献类型：
--
作者：
Dai, BS;Saada, N;Palade, P
被称为α(1C)或Ca(V)1.2的心脏钙通道表达一个新的更长的第一外显子，与以前在兔心脏或大鼠主动脉中报道的相同。核糖核酸酶保护分析表明，该外显子存在于人心脏RNA中的大多数Ca(V)1.2转录本中。该外显子的存在也表明该转录本的表达是由该外显子及其5‘非翻译区上游的启动子驱动的。推测的启动子与大鼠对应的启动子有69%的同源性，当以荧光素酶表达载体导入培养的心肌细胞时，显示出功能性启动子活性。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
The cardiac Ca channel known as alpha(1C) or Ca(V)1.2 is shown to express a new longer first exon equivalent to that formerly reported in rabbit heart or rat aorta. Ribonuclease protection assay indicates that this exon is found in the majority of Ca(V)1.2 transcripts in human heart RNA. The presence of this exon also suggests that expression of this transcript is driven by a promoter immediately upstream of this exon and its 5' untranslated region. The putative promoter exhibits 69% homology to its rat counterpart and displays functional promoter activity when transfected into heart cells in culture in luciferase-expressing constructs. (C) 2002 Elsevier Science (USA). All rights reserved.