Recent computational developments on CLIP-seq data analysis and microRNA targeting implications.
Recent computational developments on CLIP-seq data analysis and microRNA targeting implications.
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DOI：
10.1093/bib/bbx063
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发表时间：
2018-11-27
影响因子：
9.5
通讯作者：
Trabucchi M
中科院分区：
文献类型：
--
作者：
Bottini S;Pratella D;Grandjean V;Repetto E;Trabucchi M
Cross-Linking Immunoprecipitation associated to high-throughput sequencing (CLIP-seq) is a technique used to identify RNA directly bound to RNA-binding proteins across the entire transcriptome in cell or tissue samples. Recent technological and computational advances permit the analysis of many CLIP-seq samples simultaneously, allowing us to reveal the comprehensive network of RNA–protein interaction and to integrate it to other genome-wide analyses. Therefore, the design and quality management of the CLIP-seq analyses are of critical importance to extract clean and biological meaningful information from CLIP-seq experiments. The application of CLIP-seq technique to Argonaute 2 (Ago2) protein, the main component of the microRNA (miRNA)-induced silencing complex, reveals the direct binding sites of miRNAs, thus providing insightful information about the role played by miRNA(s). In this review, we summarize and discuss the most recent computational methods for CLIP-seq analysis, and discuss their impact on Ago2/miRNA-binding site identification and prediction with a regard toward human pathologies.
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影响因子：
3
作者：
Bailey TL;Bodén M;Whitington T;Machanick P
通讯作者：
Machanick P
影响因子：
4.4
作者：
Chou CH;Lin FM;Chou MT;Hsu SD;Chang TH;Weng SL;Shrestha S;Hsiao CC;Hung JH;Huang HD
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Huang HD
影响因子：
14.9
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Ahadi A;Sablok G;Hutvagner G
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Hutvagner G
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14.9
作者：
Cho S;Jang I;Jun Y;Yoon S;Ko M;Kwon Y;Choi I;Chang H;Ryu D;Lee B;Kim VN;Kim W;Lee S
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Lee S
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS