Uniaxially crumpled graphene as a platform for guided myotube formation
Uniaxially crumpled graphene as a platform for guided myotube formation
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DOI：
10.1038/s41378-019-0098-6
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发表时间：
2019-11-04
影响因子：
7.9
通讯作者：
Nam, SungWoo
中科院分区：
文献类型：
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作者：
Kim, Junghoon;Leem, Juyoung;Nam, SungWoo
石墨烯由于其固有的化学惰性、生物相容性和机械柔性，在引导细胞行为如粘附和分化方面具有巨大的潜力。然而，由于石墨烯的二维（2D）性质，将石墨烯微加工成微米/纳米级图案已被广泛用于引导细胞组装。在这项研究中，我们报告了褶皱石墨烯，即，具有纳米级波浪状表面纹理的单片限定的石墨烯，作为可以有效地促进对齐的C2 C12小鼠成肌细胞分化的组织工程平台。我们通过压缩应变诱导变形将平面外的纳米级褶皱形态赋予平坦的石墨烯。当将C2 C12小鼠成肌细胞接种在单轴褶皱石墨烯上时，不仅在单细胞水平上促进了对齐和伸长，而且与平坦石墨烯相比，肌管的分化和成熟也得到了增强。这些结果证明了褶皱石墨烯平台用于骨骼肌组织的组织工程和再生医学的实用性。
Graphene, owing to its inherent chemical inertness, biocompatibility, and mechanical flexibility, has great potential in guiding cell behaviors such as adhesion and differentiation. However, due to the two-dimensional (2D) nature of graphene, the microfabrication of graphene into micro/nanoscale patterns has been widely adopted for guiding cellular assembly. In this study, we report crumpled graphene, i.e., monolithically defined graphene with a nanoscale wavy surface texture, as a tissue engineering platform that can efficiently promote aligned C2C12 mouse myoblast cell differentiation. We imparted out-of-plane, nanoscale crumpled morphologies to flat graphene via compressive strain-induced deformation. When C2C12 mouse myoblast cells were seeded on the uniaxially crumpled graphene, not only were the alignment and elongation promoted at a single-cell level but also the differentiation and maturation of myotubes were enhanced compared to that on flat graphene. These results demonstrate the utility of the crumpled graphene platform for tissue engineering and regenerative medicine for skeletal muscle tissues.