The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline.
The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline.
复制标题
DOI：
10.2147/ijn.s15308
复制
发表时间：
2011-01-17
影响因子：
8
通讯作者：
Hashem KS
中科院分区：
文献类型：
--
作者：
Amin KA;Hassan MS;Awad el-ST;Hashem KS
本研究的目的是确定氧化铈（CeO 2）纳米粒子的能力，以防止野百合碱（MCT）诱导的肝毒性大鼠模型。将20只雄性Sprague道利大鼠随机分配至4组：对照组（接受生理盐水）、CeO 2组（腹腔内给予0.0001 nmol/kg [IP]）、MCT组（单次IP给予10 mg/kg体重）和MCT + CeO 2组（在MCT前后均接受CeO 2）。对大鼠肝脏进行电子显微镜成像，并定量肝脏总谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPX）、谷胱甘肽S-转移酶（GST）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的酶活性。结果显示，MCT诱导的总肝脏GSH、GPX、GR和GST显著降低，与同时给予CeO 2的对照值标准化。此外，MCT使肝脏CAT和SOD活性显著增加，CeO 2也可改善。这些结果表明，CeO 2作为一个公认的新的和有效的肝脏保护剂对MCT诱导的肝毒性。
The objective of the present study was to determine the ability of cerium oxide (CeO2) nanoparticles to protect against monocrotaline (MCT)-induced hepatotoxicity in a rat model. Twenty male Sprague Dawley rats were arbitrarily assigned to four groups: control (received saline), CeO2 (given 0.0001 nmol/kg intraperitoneally [IP]), MCT (given 10 mg/kg body weight IP as a single dose), and MCT + CeO2 (received CeO2 both before and after MCT). Electron microscopic imaging of the rat livers was carried out, and hepatic total glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) enzymatic activities were quantified. Results showed a significant MCT-induced decrease in total hepatic GSH, GPX, GR, and GST normalized to control values with concurrent CeO2 administration. In addition, MCT produced significant increases in hepatic CAT and SOD activities, which also ameliorated with CeO2. These results indicate that CeO2 acts as a putative novel and effective hepatoprotective agent against MCT-induced hepatotoxicity.