DIFFERENTIAL CHANGES IN THE URINARY-EXCRETION OF 2 ORALLY-ADMINISTERED POLYETHYLENE-GLYCOL MARKERS (PEG-900 AND PEG-4000) IN RATS AFTER FEEDING VARIOUS CARBOHYDRATE GELLING AGENTS
DIFFERENTIAL CHANGES IN THE URINARY-EXCRETION OF 2 ORALLY-ADMINISTERED POLYETHYLENE-GLYCOL MARKERS (PEG-900 AND PEG-4000) IN RATS AFTER FEEDING VARIOUS CARBOHYDRATE GELLING AGENTS
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DOI：
10.1093/jn/119.3.380
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发表时间：
1989-03-01
影响因子：
4.2
通讯作者：
CASPARY, WF
中科院分区：
文献类型：
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作者：
ELSENHANS, B;CASPARY, WF
在喂食各种碳水化合物胶凝剂后，测量大鼠经口给予的两种聚乙二醇标记物（PEG 900和PEG 4000）的尿排泄。将果胶、瓜尔胶、甲基纤维素和角叉菜胶添加（20%wt/wt）到无纤维对照饮食中，并在实验前喂养4周。在饮用水中给予标记物后96 h内测量3 H-PEG 900和14 C-PEG 400的排泄。一般来说，尿中PEG 4000与PEG 900的比例在纤维喂养后增加：从对照组的0.20分别增加到果胶、瓜尔胶、甲基纤维素或角叉菜胶喂养组的0.31、0.37、0.29或0.27（中位数）。在喂食无纤维饮食的大鼠中，两种PeG标记物的排泄模式与喂食多糖的大鼠不同。果胶和瓜尔胶，两种多糖容易被肠道细菌发酵，导致更高的排泄量的PEG 4000，但不变的排泄量的PEG 900，而甲基纤维素和角叉菜胶，两种多糖对微生物降解更惰性，与较低的排泄量的PEG 900与不变的排泄量的PEG 4000。这项研究表明，多糖，这可能是膳食纤维的可溶性部分的代表，可以影响较大分子的肠道渗透性。可微生物降解的多糖尤其可导致较大分子的吸收增加。
The urinary excretion of two orally administered polyethylene glycol markers (PEG 900 and PEG 4000) was measured in rats after feeding various carbohydrate gelling agents. Pectin, guaran, methylcellulose, and carrageenan were aded (20% wt/wt) to a fiber-free control diet and were fed for 4 wk prior to the experiment. Excretion of 3H-PEG 900 and 14C-PEG 400 was measured over a period of 96 h after administration of the markers in the drinking water. In general, the ratio of PEG 4000 to PEG 900 in the urine increased after fiber feeding: from 0.20 in the controls to 0.31, 0.37, 0.29 or 0.27 (medians) in the pectin-, guaran-, methylcellulose-, or carrageenan-fed group, respectively. The pattern of excretion of the two PeG markers in rats fed the fiber-free diet differed from that in the polysaccharide-fed rats. Pectin and guaran, two polysaccharides readily fermented by intestinal bacteria, led to a higher excretion of PEG 4000 but an unaltered excretion of PEG 900, whereas methylcellulose and carrageenan, two polysaccharides more inert against microbial degradation, were associated with a lower excretion of PEG 900 with unchanged excretion of PEG 4000. The study shows that polysaccharides, which may be representative of the soluble portion of dietary fiber, can influence the intestinal permeability of larger molecules. The microbiologically degradable polysaccharides in particular may lead to an increased absorption of larger molecules.