Detection of novel SNPs and mapping of the fatness QTL on pig chromosome 7q1.1-1.4 region.
Detection of novel SNPs and mapping of the fatness QTL on pig chromosome 7q1.1-1.4 region.
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DOI：
10.4238/2011.december.14.2
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
W. Huang;Z. Ma;Z. Xu;Y. Xiong;B. Zuo
中科院分区：
文献类型：
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作者：
W. Huang;Z. Ma;Z. Xu;Y. Xiong;B. Zuo
在不同的猪资源群体中，已经定位了多个与肥度性状相关的QTL，定位于猪染色体7q1.1-1.4。对8个新标记进行了基因分型，包括BTNL 1、COL 21 A1、PPARD、GLP 1 R、MDFI、GNMT、ABCC 10和PLA 2G 7基因中的7个SNP和1个插入或缺失，以及SLC 39 A7和HMGA 1基因中的2个SNP。梅山猪与大白色猪之间除了HMGA 1和ABCC 10基因的2个SNP外，其余8个标记的等位基因频率差异均达到极显著水平。利用8个多态性位点对大白色×梅山F2资源群体进行连锁分析和QTL定位，以确定肥度QTL。共检测到5个染色体上显著的QTL，其中叶脂重、6- 7肋背膘厚和平均背膘厚的QTL位于SSC 7p1.1-q1.4上PPARD和GLP 1 R基因之间，胸腰背膘厚的QTL位于SSC 7p1.1-q1.4上GNMT和PLA 2G 7基因之间。
Many QTLs for fatness traits have been mapped on pig chromosome 7q1.1-1.4 in various pig resource populations. Eight novel markers, including seven SNPs and one insertion or deletion within BTNL1, COL21A1, PPARD, GLP1R, MDFI, GNMT, ABCC10, and PLA2G7 genes, as well as two previously reported SNPs in SLC39A7 and HMGA1 genes, were genotyped in Large White and Meishan pig breeds. Except for two SNPs in HMGA1 and ABCC10 genes, allele frequencies of the other eight markers are highly significant different between Chinese indigenous Meishan breeds and Large White pig breeds. Eight polymorphic sites were then used for linkage and QTL mapping to refine the fatness QTL in a Large White × Meishan F(2) resource population. Five chromosome-wise significant QTLs were detected, of which the QTLs for leaf fat weight, backfat thickness at 6-7th rib and rump, and mean backfat thickness were narrowed to the interval between PPARD and GLP1R genes and the QTL for backfat thickness at thorax-waist between GNMT and PLA2G7 genes on SSC7p1.1-q1.4.