New classification system for oxygenase components involved in ring-hydroxylating oxygenations
New classification system for oxygenase components involved in ring-hydroxylating oxygenations
复制标题
DOI：
10.1271/bbb.65.254
复制
发表时间：
2001-02-01
影响因子：
1.6
通讯作者：
Omori, T
中科院分区：
文献类型：
--
作者：
Nam, JW;Nojiri, H;Omori, T
Batie等人[黄酶的化学与生物化学，3,543 -556(1991)]提出了一种环羟化加氧酶的分类系统，根据组成成分的数量和氧化还原中心的性质将加氧酶分为三类。但近年来，许多环羟基化加氧酶被新发现和表征，很难将其归为这三类。典型的例子是咔唑1,9a-双加氧酶和2-氧-1,2-二氢喹啉8-单加氧酶，从生化特性上分别被划分为Hi类和IB类。然而，系统发育研究表明，两者的末端加氧酶与IA类密切相关。由于这种差异来自于将所有组分一起计算，因此我们提出了一种基于末端加氧酶组分α亚基氨基酸序列同源性的新方案。这一新方案强烈反映了末端加氧酶组分的实际系统发育关系。通过CLUSTAL W程序对54个加氧酶组分进行序列比较，将其分为4组（I、II、III和IV组），其中I组含范围较广且同源性较低的加氧酶，II、Hi和IV组含有一些典型的加氧酶：苯甲酸酯/甲苯酸酯双加氧酶为II组，萘/多环芳烃双加氧酶为Hi组，苯/甲苯/联苯双加氧酶为IV组。我们的新方案简单而强大，因为当DNA序列可用时，加氧酶成分几乎可以自动分组，并且它与系统发育关系非常吻合。
Batie et al. [Chemistry and Biochemistry of Flavoenzymes, 3, 543-556 (1991)] proposed a classification system for ring-hydroxylating oxygenases in which the oxygenases are grouped into three classes in terms of the number of constituent components and the nature of the redox centers. But in recent gears, many ring-hydroxylating oxygenases have been newly identified and characterized, and found difficult to classify into these three classes. Typical examples are carbazole 1,9a-dioxygenase and 2-oxo-1,2-dihydroquinoline 8-monooxygenase, which have been classified into class Hi and class IB, respectively, from biochemical characteristics. However, a phylogenetic study showed that the terminal oxygenases of both are closely related to class IA. Because this discrepancy derived from counting all the components together, here we proposed a new scheme based on the homology of the amino acid sequences of the alpha subunits of the terminal oxygenase components. This new scheme strongly reflects the actual phylogenetic affiliation of the terminal oxygenase component. By comparing their sequences painwise using the CLUSTAL W program, 54 oxygenase components were classified into 4 groups (groups I, II, III, and IV). while group I contains broad-range oxygenases sharing low homology, groups II, Hi, and IV contain some typical oxygenases: benzoate/toluate dioxygenases for group II, naphthalene/polycyclic aromatic hydrocarbon dioxygenases for group Hi, and benzene/toluene/biphenyl dioxygenases for group IV. Our new scheme is simple and powerful, since an oxygenase component can be nearly automatically grouped when the DNA sequence Is available, and it fits very wed with the phylogenetic affiliation.