Inheritance of self-compatibility and flower morphology in an inter-specific buckwheat hybrid
Inheritance of self-compatibility and flower morphology in an inter-specific buckwheat hybrid
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DOI：
10.4141/p98-117
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发表时间：
1999-10-01
影响因子：
1.2
通讯作者：
Campbell, CG
中科院分区：
文献类型：
--
作者：
Woo, SH;Adachi, T;Campbell, CG
本研究以野荞麦（Fagopyrumhomotropicum）为材料，通过与普通荞麦（F. buckingham）杂交，研究了野荞麦自交亲和性和同型花型的遗传规律。esculentum）。栽培番茄种间杂交种。（普通荞麦）及其野生近缘种F.经控制授粉和胚挽救培养后，产生了均温性植株。以穗型荞麦为母本比以针型荞麦为母本杂交亲和性好。所得F-1植株部分可育，晚熟，花形和株高介于双亲之间。它们分离成异花柱型和同型花柱型，数量相等，表明同型花柱由一对显性基因控制。以普通荞麦为材料，利用胚挽救培养技术，对甜荞F1代进行回交。通过自交将同型花柱杂种F-1推进到F-2和F-3代，并与BC 1F 1一起用于花柱基因的分析。研究结果表明，编码花柱异型和花柱同型的基因受复等位基因S控制。F. esculentum由单个遗传基因座S控制，该基因座具有两个等位基因S和s，这两个等位基因分别控制Ss（thrum-type）以及ss（pin-type）植物中的反应。同形花型关闭。homotropicum由等位基因S-h控制。这些基因可以通过显性关系来表征，即S > S-h > s。F.通过种子蛋白电泳分析，证实了homotropicum基因导入普通荞麦。
This study was conducted to determine the inheritance of self-compatibility and homomorphic flower type when the wild species Fagopyrum homotropicum was crossed with common buckwheat (F. esculentum). Unidirectional interspecific hybrids between cultivated F, esculentum Moench. (common buckwheat) and its wild relative F. homotropicum were produced after controlled pollination and embryo rescue culture. Cross-compatibility was found to be better when thrum-type common buckwheat was used as the female parent rather than the pin-type. The resulting F-1 plants were partially fertile, late maturing and intermediate between the parents in flower shape and plant height. They segregated into heterostylic (thrum only) and homostylic types in equal numbers, indicating that homostyly is controlled by a single dominant gene. The thrum-type F-1 hybrids were backcrossed to common buckwheat and the progenies were raised utilizing embryo rescue culture. The homostylic F-1 hybrids were advanced to the F-2 and F-3 generations through self-fertilization and utilized, together with the BC1F1, for the analysis of the stylar genes. The results obtained indicate that the genes coding for heterostyly and homostyly are controlled by the multiple allelic gene S. It appears that the pin/thrum complex in F. esculentum is governed by a single genetic locus S with two alleles S and s that control the reaction in Ss (thrum-type) as well as the ss (pin-type) plants, respectively. The homomorphic flower type off. homotropicum is governed by the allele S-h. These genes can be characterized by a relationship of dominance, i.e. S > S-h > s. The introgression of F. homotropicum genes into common buckwheat was verified by means of electrophoretic analysis of seed proteins.