Host RNAs, including transposons, are encapsidated by a eukaryotic single-stranded RNA virus
Host RNAs, including transposons, are encapsidated by a eukaryotic single-stranded RNA virus
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DOI：
10.1073/pnas.1116168109
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发表时间：
2012-02-07
影响因子：
11.1
通讯作者：
Johnson, John E.
中科院分区：
文献类型：
--
作者：
Routh, Andrew;Domitrovic, Tatiana;Johnson, John E.
下一代测序是我们日益了解病毒群体遗传多样性的一个有价值的工具。使用这种技术，我们已经研究了纯化的无包膜单链RNA病毒，鸡舍病毒（FHV）的RNA含量。我们还研究了病毒样颗粒（VLP）的FHV和相关的Nudaurelia capensis omega病毒的RNA含量。VLP主要包装核糖体RNA及其杆状病毒表达载体的转录物。此外，我们发现5.3%的包装RNA是来自Sf21基因组的转座因子。在考虑VLP的治疗用途时，这一观察结果可能很重要。我们发现，真正的FHV病毒粒子也包装各种宿主RNA，占包装核酸的1%。大量的宿主信使RNA、核糖体RNA、非编码RNA和转座因子很容易被检测到。这些宿主RNA的包装使得共享病毒病原体的真核宿主之间的水平基因转移成为可能。我们的结论是，无包膜RNA病毒的遗传内容是可变的，不仅通过基因组突变，而且在包装的RNA转录本的多样性。
Next-generation sequencing is a valuable tool in our growing understanding of the genetic diversity of viral populations. Using this technology, we have investigated the RNA content of a purified nonenveloped single-stranded RNA virus, flock house virus (FHV). We have also investigated the RNA content of virus-like particles (VLPs) of FHV and the related Nudaurelia capensis omega virus. VLPs predominantly package ribosomal RNA and transcripts of their baculoviral expression vectors. In addition, we find that 5.3% of the packaged RNAs are transposable elements derived from the Sf21 genome. This observation may be important when considering the therapeutic use of VLPs. We find that authentic FHV virions also package a variety of host RNAs, accounting for 1% of the packaged nucleic acid. Significant quantities of host messenger RNAs, ribosomal RNA, noncoding RNAs, and transposable elements are readily detected. The packaging of these host RNAs elicits the possibility of horizontal gene transfer between eukaryotic hosts that share a viral pathogen. We conclude that the genetic content of nonenveloped RNA viruses is variable, not just by genome mutation, but also in the diversity of RNA transcripts that are packaged.