SUBCELLULAR-LOCALIZATION, REDISTRIBUTION AND PHOTOBLEACHING OF SULFONATED ALUMINUM PHTHALOCYANINES IN A HUMAN-MELANOMA CELL-LINE
SUBCELLULAR-LOCALIZATION, REDISTRIBUTION AND PHOTOBLEACHING OF SULFONATED ALUMINUM PHTHALOCYANINES IN A HUMAN-MELANOMA CELL-LINE
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DOI：
10.1002/ijc.2910490225
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发表时间：
1991-09-09
影响因子：
6.4
通讯作者：
NESLAND, JM
中科院分区：
文献类型：
--
作者：
PENG, Q;FARRANTS, GW;NESLAND, JM
应用激光共聚焦显微镜和图像处理技术，研究了磺化铝酞菁荧光衍生物（Al-PcS（n），n = 1-4）在人黑色素瘤细胞（LOX）中的定位、移位和光漂白。使用共聚焦显微镜允许获得三维信息。Al-PcS 1和Al-PcS 2均弥散分布于细胞质中，而Al-PcS 3和Al-PcS 4则呈颗粒状分布于细胞核内。在细胞核中没有观察到Al-PcS（n）家族，除了在用Al-PcS 1和Al-PcS 2处理的一些细胞的细胞核中偶尔检测到一小部分荧光。此外，在最初与Al-PcS 3（或Al-PcS 4）孵育后，随后与从细胞溶酶体发出红色荧光的吖啶橙子（AO）孵育，在相同细胞中发现完全相同的颗粒定位模式和位置。因此，Al-PcS 3和Al-PcS 4的颗粒荧光被限制在LOX细胞的溶酶体中。非致死性的激光曝光的细胞孵育高浓度的2染料导致的染料从溶酶体的易位到整个细胞质和总细胞内荧光强度的增加。最后，一小部分染料定位到细胞核中。与低浓度的溶酶体定位染料孵育的细胞的激光曝光导致细胞内荧光强度增加，而染料没有移位。在所有条件下，高激光照射导致细胞内总荧光强度降低。
Intracellular localization, intracellular translocation and photobleaching following non-lethal laser microirradiation of the fluorescing derivatives of sulfonated aluminum phthalocyanines (Al-PcS(n), n = 1-4) in a human melanoma cell line (LOX) were studied by means of confocal laser scanning microscopy (LSM) and image processing. Use of confocal microscopy allowed 3-dimensional information to be obtained. Both Al-PcS1 and Al-PcS2 localized diffusely in the cytoplasm of the cells, while Al-PcS3 and Al-PcS4 exhibited a granular pattern in the extranuclear fraction of the cells. None of the Al-PcS(n) family was observed in the nuclei of the cells except that a small fraction of fluorescence was occasionally detected in nuclei of some cells treated with Al-PcS1 and Al-PcS2. Furthermore, exactly the same granular localization patterns and positions in the same cells were found after incubation initially with Al-PcS3 (or Al-PcS4) followed by acridine orange (AO) which emits red fluorescence from lysosomes of cells. Thus, the granular fluorescence of Al-PcS3 and Al-PcS4 is confined to the lysosomes of the LOX cells. Non-lethal laser exposure of cells incubated with high concentrations of the 2 dyes resulted in a translocation of the dyes from the lysosomes to the whole cytoplasm and an increase in total intracellular fluorescence intensity. Finally, a small fraction of the dyes localized into the nuclei of the cells. The laser exposure of cells incubated with low concentrations of the lysosomally localized dyes resulted in an increase in the intracellular fluorescence intensity with no translocation of the dyes. Under all conditions, high laser exposure resulted in a decrease in the total intracellular fluorescence intensity.