Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection
Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection
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DOI：
10.1099/vir.0.013482-0
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发表时间：
2010-01-01
影响因子：
3.8
通讯作者：
Kimura, Hiroshi
中科院分区：
文献类型：
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作者：
Iwata, Seiko;Wada, Kaoru;Kimura, Hiroshi
慢性活动性EB病毒（CAEBV）感染是一种系统性EB病毒（EBV）阳性淋巴组织增生性疾病，其特征是在无已知免疫缺陷的患者中出现持续或复发的传染性单核细胞增多症样症状。该疾病的详细发病机制尚不清楚，也没有标准的治疗方案。对24例CAEBV感染者外周血标本进行EBV基因表达分析。通过实时RT-PCR定量6个潜伏性和2个裂解性EBV基因的表达水平。EBV编码的小RNA 1和BamHI-A转录本在所有患者中均大量检测到，并且在大多数患者中观察到潜伏膜蛋白（LMP）2。EBV核抗原（EBNA）1和LMP 1检测频率较低，表达水平较低。EBNA 2和两个裂解基因在任何患者中均未检测到。潜伏基因表达的模式被确定为潜伏II型。EBNA 1在临床活动性患者中检测频率更高，水平更高。量化EB病毒基因表达有助于阐明CAEB病毒感染的发病机制，并可以提供有关患者疾病预后以及可能的治疗干预措施的信息。
Chronic active Epstein-Barr virus (CAEBV) infection is a systemic Epstein-Barr virus (EBV)-positive lymphoproliferative disorder characterized by persistent or recurrent infectious mononucleosis-like symptoms in patients with no known immunodeficiency. The detailed pathogenesis of the disease is unknown and no standard treatment regimen has been developed. EBV gene expression was analysed in peripheral blood samples collected from 24 patients with CAEBV infection. The expression levels of six latent and two lytic EBV genes were quantified by real-time RT-PCR. EBV-encoded small RNA 1 and BamHI-A rightward transcripts were abundantly detected in all patients, and latent membrane protein (LMP) 2 was observed in most patients. EBV nuclear antigen (EBNA) 1 and LMP1 were detected less frequently and were expressed at lower levels. EBNA2 and the two lytic genes were not detected in any of the patients. The pattern of latent gene expression was determined to be latency type II. EBNA1 was detected more frequently and at higher levels in the clinically active patients. Quantifying EBV gene expression is useful in clarifying the pathogenesis of CAEBV infection and may provide information regarding a patient's disease prognosis, as well as possible therapeutic interventions.