Runx1 function in hematopoiesis is required in cells that express Tek
Runx1 function in hematopoiesis is required in cells that express Tek
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DOI：
10.1182/blood-2005-05-1955
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发表时间：
2006-01-01
期刊：
影响因子：
20.3
通讯作者：
Speck, NA
中科院分区：
文献类型：
--
作者：
Li, Z;Chen, MJ;Speck, NA
Runx1表达标志着小鼠妊娠的胚胎天（E）8.5至11.5之间的推定的生血内皮，并且是主动脉内造血簇形成所需的，导致Runx1是从内皮细胞向造血细胞转变所需的假设。为了解决这一假设，我们消融Runx1基因的Cre重组酶介导的切除，与Cre表达的控制下的Tek启动子和增强子。大多数胚胎在E12.5和El之间死亡，具有几乎相同的Runx1缺陷表型。我们的结论是Runx1功能建立明确的造血是需要在一个Tek（+）细胞。
Runx1 expression marks the putative hemogenic endothelium between embryonic days (E) 8.5 to 11.5 of mouse gestation and is required for the formation of intra-aortic hematopoietic clusters, leading to the hypothesis that Runx1 is required for the transition from endothelial to hematopoietic cell. To address this hypothesis, we ablated the Runx1 gene by Cre-recombinase-mediated excision, with Cre expression under the control of the Tek promoter and enhancer. Most embryos died between E12.5 and El with a phenotype almost identical Runx1 deficiency. We conclude that Runx1 function in establishing definitive hematopoiesis is required in a Tek(+) cell.