ROS implication in a new antitumor strategy based on non-thermal plasma
ROS implication in a new antitumor strategy based on non-thermal plasma
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DOI：
10.1002/ijc.26252
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发表时间：
2012-05-01
影响因子：
6.4
通讯作者：
Le Pape, Alain
中科院分区：
文献类型：
--
作者：
Vandamme, Marc;Robert, Eric;Le Pape, Alain
非热等离子体（NTP）是通过电离中性气体分子/原子产生的，导致在环境温度下含有激发分子、反应物质的高反应性气体并产生瞬态电场。鉴于其与组织或细胞相互作用而不显著升高温度的潜力，NTP似乎是治疗包括癌症在内的各种疾病的有希望的方法。我们研究的目的是评估NTP在体外和体内的作用。为此，我们评估了NTP对两种人类癌细胞系（胶质母细胞瘤U87 MG和结直肠癌HCT-116）的体外抗肿瘤活性。我们的数据表明，NTP产生大量的活性氧（ROS），导致DNA损伤的形成。这导致多相细胞周期停滞和随后的凋亡诱导。此外，在U87 MG荷瘤小鼠的体内实验表明，与未处理的小鼠相比，NTP诱导生物发光和肿瘤体积的减少。还观察到细胞凋亡的诱导以及细胞在细胞周期的S期的积累，表明肿瘤增殖的停滞。总之，我们在这里证明了NTP产生ROS的潜力使得这种策略在肿瘤治疗的背景下特别有前途。
Non-thermal plasma (NTP) is generated by ionizing neutral gas molecules/atoms leading to a highly reactive gas at ambient temperature containing excited molecules, reactive species and generating transient electric fields. Given its potential to interact with tissue or cells without a significant temperature increase, NTP appears as a promising approach for the treatment of various diseases including cancer. The aim of our study was to evaluate the interest of NTP both in vitro and in vivo. To this end, we evaluated the antitumor activity of NTP in vitro on two human cancer cell lines (glioblastoma U87MG and colorectal carcinoma HCT-116). Our data showed that NTP generated a large amount of reactive oxygen species (ROS), leading to the formation of DNA damages. This resulted in a multiphase cell cycle arrest and a subsequent apoptosis induction. In addition, in vivo experiments on U87MG bearing mice showed that NTP induced a reduction of bioluminescence and tumor volume as compared to nontreated mice. An induction of apoptosis was also observed together with an accumulation of cells in S phase of the cell cycle suggesting an arrest of tumor proliferation. In conclusion, we demonstrated here that the potential of NTP to generate ROS renders this strategy particularly promising in the context of tumor treatment.