Non-invasive auditory brainstem responses to FM sweeps in awake big brown bats.

Non-invasive auditory brainstem responses to FM sweeps in awake big brown bats.
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醒着的大棕色蝙蝠对FM扫描的非侵入性听觉脑干反应。

DOI:
10.1007/s00359-022-01559-w
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发表时间:
2022-07
期刊:
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
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其他
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我们介绍了两种脑电图技术,一个基于传统的单极电极和一个基于一种新的三极电极,记录的第一次听觉脑干反应(ABR)从头皮的未麻醉,无拘无束的大棕蝙蝠。刺激是调频(FM)扫描,扫描方向、扫描持续时间和谐波结构各不相同。正如先前的侵入性ABR记录所预期的那样,向上扫描FM信号比向下扫描FM信号诱发更大的振幅响应(刺激开始后3-5 ms的潜伏期范围内的峰谷振幅)。头皮记录的反应显示的振幅-潜伏期交易的影响,从侵入性的录音预期。这两个发现证实了我们的无创记录技术的可靠性。非侵入性记录在未麻醉,不受限制的蝙蝠的可行性,将激励未来的研究发现在这些动物的生物声纳信号的感知和认知处理的电生理特征,并允许更好地比较与ABR数据从回声定位鲸类,侵入性实验受到严重限制。
We introduce two EEG techniques, one based on conventional monopolar electrodes and one based on a novel tripolar electrode, to record for the first time auditory brainstem responses (ABRs) from the scalp of unanesthetized, unrestrained big brown bats. Stimuli were frequency-modulated (FM) sweeps varying in sweep direction, sweep duration, and harmonic structure. As expected from previous invasive ABR recordings, upward-sweeping FM signals evoked larger amplitude responses (peak-to-trough amplitude in the latency range of 3–5 ms post-stimulus onset) than downward-sweeping FM signals. Scalp-recorded responses displayed amplitude-latency trading effects as expected from invasive recordings. These two findings validate the reliability of our noninvasive recording techniques. The feasibility of recording noninvasively in unanesthetized, unrestrained bats will energize future research uncovering electrophysiological signatures of perceptual and cognitive processing of biosonar signals in these animals, and allows for better comparison with ABR data from echolocating cetaceans, where invasive experiments are heavily restricted.
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