Genetic screens for mutations affecting development of Xenopus tropicalis
Genetic screens for mutations affecting development of Xenopus tropicalis
复制标题
DOI：
10.1371/journal.pgen.0020091
复制
发表时间：
2006-06-01
期刊：
影响因子：
4.5
通讯作者：
Zimmerman, Lyle B.
中科院分区：
文献类型：
--
作者：
Goda, Tadahiro;Abu-Daya, Anita;Zimmerman, Lyle B.
我们在这里提出的结果正向和反向遗传筛选化学诱导的突变非洲爪蟾热带。在我们的正向遗传筛选中，我们发现了77种不同器官发生和分化过程中的候选表型。使用雌核发育筛选设计，最大限度地减少时间和饲养空间支出，我们发现，如果一个表型被检测到在雌核发育F2的给定F1女性两次，它很可能是一个遗传异常（29/29例）。我们还证明了反向遗传方法获得特定基因突变载体的可行性，并通过对诱变群体中特定外显子进行测序直接确定了诱导突变率。非洲爪蟾系统，其良好的理解胚胎学，命运图，和获得的功能的方法，现在可以再加上有效的功能丧失的遗传策略，脊椎动物功能基因组学和发育遗传学。
We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.