Cellular structural biology
Cellular structural biology
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DOI：
10.1016/j.sbi.2010.07.006
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发表时间：
2010-10-01
影响因子：
6.8
通讯作者：
Selenko, Philipp
中科院分区：
文献类型：
--
作者：
Ito, Yutaka;Selenko, Philipp
虽然我们认识到细胞内环境的复杂性是每个生物体的一般特性，但我们共同缺乏适当的工具来分析细胞内蛋白质结构，细胞内核磁共振波谱代表了一种新的生物物理工具，可以在活细胞内的原子水平上研究生物分子的构象和功能特征。我们回顾了细胞内核磁共振的最新发展，并对未来在原核和真核细胞中的应用进行了展望。我们希望借此强调细胞内核磁共振技术在复杂生物过程的细胞研究和天然环境中结构分析的有用性
While we appreciate the complexity of the intracellular environment as a general property of every living organism, we collectively lack the appropriate tools to analyze protein structures in a cellular context In-cell NMR spectroscopy represents a novel biophysical tool to investigate the conformational and functional characteristics of biomolecules at the atomic level inside live cells Here, we review recent in-cell NMR developments and provide an outlook towards future applications in prokaryotic and eukaryotic cells We hope to thereby emphasize the usefulness of in-cell NMR techniques for cellular studies of complex biological processes and for structural analyses in native environments