Identification of zona binding proteins of rabbit, pig, human, and mouse spermatozoa on nitrocellulose blots.
Identification of zona binding proteins of rabbit, pig, human, and mouse spermatozoa on nitrocellulose blots.
复制标题
在硝酸纤维素印迹上鉴定兔、猪、人和小鼠精子的透明带结合蛋白。
DOI：
10.1002/jez.1402350314
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Fisher,SJ
中科院分区：
文献类型：
--
作者：
O'Rand,MG;Matthews,JE;Welch,JE;Fisher,SJ
哺乳动物受精是一个多步骤的过程，每个步骤对特异性的要求都不同。在本报告中，我们研究了精子的结合同源和异源透明带。本文报道了家兔、猪、人精子和小鼠附睾精子中的同源ZBP。兔的ZBP具有32 K、18 K、16 K和14 K的相对分子质量（MW）;猪的主要ZBP是16 K，而人精子在17 K和18 K结合人精子蛋白。小鼠精子的ZBP是19 K、18 K和16 K。
Mammalian fertilization is a multi‐step process with different requirements for specificity at each step. In the present report we have examined the binding of spermatozoa to homologous and heterologous zonae pellucidae. The homologous zona binding proteins (ZBP) of ejaculated rabbit, pig and human spermatozoa and epididymal mouse spermatozoa have been identified. The rabbit's ZBPs have relative molecular weights (MW) of 32K, 18K, 16K and 14K; the pig's major ZBP is 16K while human spermatozoa bind human zona protein at 17K and 18K. Mouse sperm ZBPs are 19K, 18K and 16K.