The Ozobranchus leech is a candidate mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas)
The Ozobranchus leech is a candidate mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas)
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DOI：
10.1016/j.virol.2003.12.026
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发表时间：
2004-03-30
期刊：
影响因子：
3.7
通讯作者：
Casey, JW
中科院分区：
文献类型：
--
作者：
Greenblatt, RJ;Work, TM;Casey, JW
海龟纤维乳头瘤病(FP)是一种引起生态关注的肿瘤性疾病。纤维乳头状瘤相关的海龟疱疹病毒(FPTHV)一直存在，通常每个肿瘤细胞的载量超过一个病毒副本。用定量聚合酶链式反应(QPCR)检测了一组绿海龟寄生虫的DNA，以确定任何携带的病毒载量是否足以证明它们是FPTHV的载体。海洋水蚤(Ozobranchus spp.)被发现携带高病毒DNA；一些样本接近每条水蚤1000万份拷贝。同源蔗糖密度梯度/qPCR分析证实，其中一些拷贝与被膜病毒密度的颗粒有关。这些数据表明，海洋水蚤Ozobranchus是FPTHV的机械媒介。FPTHV基因表达的定量RT-PCR分析表明，FPTHV在纤维乳头状瘤中的大部分拷贝具有限制性的DNA聚合酶表达，提示存在潜伏感染。(C)2004 Elsevier Inc.保留所有权利。
Fibropapillomatosis (FP) of marine turtles is a neoplastic disease of ecological concern. A fibropapilloma-associated turtle herpesvirus (FPTHV) is consistently present, usually at loads exceeding one virus copy per tumor cell. DNA from an array of parasites of green turtles (Chelonia mydas) was examined with quantitative PCR (qPCR) to determine whether any carried viral loads are sufficient to implicate them as vectors for FPTHV. Marine leeches (Ozobranchus spp.) were found to carry high viral DNA loads; some samples approached 10 million copies per leech. lsopycnic sucrose density gradient/qPCR analysis confirmed that some of these copies were associated with particles of the density of enveloped viruses. The data implicate the marine leech Ozobranchus as a mechanical vector for FPTHV. Quantitative RT-PCR analysis of FPTHV gene expression indicated that most of the FPTHV copies in a fibropapilloma have restricted DNA polymerase expression, suggestive of latent infection. (C) 2004 Elsevier Inc. All rights reserved.