NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
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NanoSOSG：用于检测细胞内单线态氧的纳米结构荧光探针
DOI：
10.1002/ange.201609050
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发表时间：
2017
影响因子：
--
通讯作者：
Ruiz-González R
中科院分区：
文献类型：
--
作者：
Ruiz-González R
设计、合成并表征了用于生物系统中的单线态氧（1 O2）检测的生物相容性荧光纳米探针，其规避了分子探针单线态氧传感器绿色®（SOSG）的许多限制。这种广泛使用的商业单线态氧探针共价连接到聚丙烯酰胺纳米颗粒核心使用不同的架构，以优化响应1 O2。与其分子对应物相反，最佳的基于SOSG的纳米探针，我们称之为NanoSOSG，很容易被E内化。大肠杆菌，不与牛血清白蛋白相互作用。此外，光谱特性在细胞内不发生变化，并且探针响应于细胞内产生的1 O2而增加荧光。
A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green®(SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to1O2. In contrast to its molecular counterpart, the optimum SOSG‐based nanoprobe, which we call NanoSOSG, is readily internalized byE. colicells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated1O2with an increase in fluorescence.