Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy
Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy
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金黄色葡萄球菌与鼠细胞因子测定的交叉反应性：差异的来源
DOI：
10.1016/j.cyto.2016.03.007
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发表时间：
2016
期刊：
影响因子：
3.8
通讯作者：
Guangxun Meng
中科院分区：
文献类型：
--
作者：
Numan Javed;Guang Xue;Ailing Lu;Yue Xing;Yoichiro Iwakura;Hui Xiao;Hervé Lecoeur;Gerald F. Späth;Guangxun Meng
金黄色葡萄球菌是革兰氏阳性菌中的一种，可引起多种疾病。在攻击后，宿主免疫细胞识别S。并产生多种免疫应答，包括产生促炎细胞因子如IL-1β和TNF-α。这些细胞因子是炎症的重要介质，其可以通过各种免疫学方法如酶联免疫吸附测定（ELISA）和免疫印迹来检测。在目前的研究中，我们发现一些临床分离株以及实验室菌株的S。金黄色葡萄球菌与ELISA抗体对小鼠IL-1β和TNF-α的检测具有交叉反应性。这种交叉反应性产生夸大的假阳性信号，这可能是理解针对S的真实的免疫应答的差异来源。宿主免疫细胞的感染。
Staphylococcus aureusis one of the versatile Gram positive bacteria causing a range of diseases. Upon challenge, host immune cells recognizeS. aureusand mount diverse immune responses including production of pro-inflammatory cytokines such as IL-1β and TNF-α. These cytokines are important mediators of inflammation which can be detected via various immunological methods such as enzyme linked immunosorbent assay (ELISA) and immunoblotting. In the current study, we found that a number of clinical isolates as well as laboratory strains ofS. aureusexhibited cross reactivity with ELISA antibodies for murine IL-1β and TNF-α assays. This cross reactivity generates exaggerated false positive signals which can be a source of discrepancy for the understanding of real immune responses againstS. aureusinfection by host immune cells.