A cycloartenol synthase from the steroidal saponin biosynthesis pathway of Paris polyphylla
A cycloartenol synthase from the steroidal saponin biosynthesis pathway of Paris polyphylla
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DOI：
10.1080/10286020.2020.1730331
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发表时间：
2020-03-05
影响因子：
1.7
通讯作者：
Li, Jia
中科院分区：
文献类型：
--
作者：
Guo, Si-Yuan;Yin, Yan;Li, Jia
Steroidal saponins named polyphyllin are the major active components of Paris polyphylla. Cycloartenol synthase (CAS) is a key enzyme that catalyzes the formation of the sterol scaffold. In this study, we cloned a putative CAS gene from Paris polyphylla. Heterologous expression in yeast indicated that PpCAS can convert 2,3-oxidosqualene into cycloartenol. qRT-PCR analysis showed that the expression of PpCAS was highest in leaves and lowest in roots. To our best knowledge, this is the first report of the functional characterization of cycloartenol synthase from Paris polyphylla, which lays the foundation for further analysis of the biosynthesis pathway of polyphyllins.
