The outer membrane of yeast mitochondria: isolation of outside‐out sealed vesicles
The outer membrane of yeast mitochondria: isolation of outside‐out sealed vesicles
复制标题
酵母线粒体外膜：外向密封囊泡的分离
作者：
H. Riezman;Rick Hay;S. Gasser;G. Daum;Greti Schneider;Christine Witte;G. Schatz
分离了酵母线粒体外膜，鉴定了其10个主要多肽(摩尔分数)。WTS。109、70、57、45、45、42、33、29、25和14kd)。膜上没有与线粒体内膜或粗面微体相同的主要多肽。酶处理和免疫化学技术表明，几乎所有分离的外膜小泡都是封闭的，并显示出与完整线粒体相同的表面取向。
The yeast mitochondrial outer membrane was isolated and 10 of its major polypeptides were identified (mol. wts. 109, 70, 57, 45, 45, 42, 33, 29, 25 and 14 kd). The membrane has no major polypeptide in common with either mitochondrial inner membrane or rough microsomes. Protease treatment and immunochemical techniques showed that virtually all of the isolated outer membrane vesicles are sealed and display the same surface orientation as in the intact mitochondrion.