WIMP, A DOMINANT MATERNAL-EFFECT MUTATION, REDUCES TRANSCRIPTION OF A SPECIFIC SUBSET OF SEGMENTATION GENES IN DROSOPHILA
WIMP, A DOMINANT MATERNAL-EFFECT MUTATION, REDUCES TRANSCRIPTION OF A SPECIFIC SUBSET OF SEGMENTATION GENES IN DROSOPHILA
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DOI：
10.1101/gad.5.3.341
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发表时间：
1991-03-01
影响因子：
10.5
通讯作者：
ISHHOROWICZ, D
中科院分区：
文献类型：
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作者：
PARKHURST, SM;ISHHOROWICZ, D
Wimp是一种显性母性效应突变，它与早期作用的母性和合子果蝇基因的特定子集相互作用。我们发现wimp是一种功能改变突变，等位于RNA聚合酶140-kD亚单位的突变，后者导致相互作用基因转录减少。不与wimp相互作用的基因座在正常水平表达。我们从转录因子和RNA聚合酶之间的特定相互作用的角度讨论了这些结果。来自懦弱母亲的胚胎显示出没有改变的命运图，并正常发育，尽管转录水平至少降低了两倍。我们认为，空间线索是由片段基因产物的平衡决定的，而不是它们的绝对浓度。我们还展示了对分割、性别决定和其他早期功能所需的其他未检测到的基因座的强大遗传筛查。
wimp is a dominant maternal-effect mutation that interacts with a specific subset of early-acting maternal and zygotic Drosophila genes. We show that wimp is a change-of-function mutation, allelic to mutations of the 140-kD subunit of RNA polymerase, which causes reduced transcription of interacting genes. Loci that do not interact with wimp are expressed at normal levels. We discuss these results in terms of specific interactions between transcription factors and RNA polymerase. Embryos from wimp mothers show unaltered fate maps and develop normally, despite the reduction of transcript levels at least twofold. We suggest that spatial cues are determined by a balance of segmentation gene products rather than their absolute concentrations. We also demonstrate powerful genetic screens for otherwise undetected loci required for segmentation, sex determination, and other early functions.