Understanding the molecular mechanisms underlying graft success in grapevine
Understanding the molecular mechanisms underlying graft success in grapevine
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DOI：
10.1186/s12870-019-1967-8
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发表时间：
2019-09-11
影响因子：
5.3
通讯作者：
Fevereiro, P.
中科院分区：
文献类型：
--
作者：
Assuncao, M.;Santos, C.;Fevereiro, P.
嫁接是一种密集的商业实践，需要保护欧洲葡萄免受根瘤蚜虫害。对这种害虫具有抗性的砧木是与欧洲葡萄品种具有不同程度相容性的美洲葡萄品种的杂交品种。为了了解葡萄藤嫁接相容性的驱动因素，采用转录组学方法寻找嫁接成功的主要调控因子。在苗圃嫁接条件下，分别在嫁接后21天和80天对两种不同亲和性水平的接穗/砧木组合进行了比较。结果在最相容的组合中，代谢和激素信号通路基因的表达较早且较高，而酚代谢和氧化应激反应基因的表达较低。移植后80天，在最相容的组合下，调节血管维持、分化和增殖的转录因子的表达较高。此外，与亲和性较差的组合相比，亲和性较好的组合中潜在靶向与植物发育相关的重要转录因子的microrna表达水平较低。结论在这种情况下，选择一组调节因子作为早期预测相容嫁接的潜在表达标记。
Background Grafting is an intensive commercial practice required to protect the European grapevine against the Phylloxera pest. Rootstocks resistant to this pest are hybrids of American vine species with different levels of compatibility with European Vitis vinifera varieties. Aiming to understand what drives grafting compatibility in grapevine, a transcriptomic approach was used to search for master regulators of graft success. Two scion/rootstock combinations, with different levels of compatibility, were compared in a nursery-grafting context at two stages, at 21 and 80 days after grafting. Results In the most compatible combination, an earlier and higher expression of genes signaling the metabolic and hormonal pathways as well as a reduced expression of genes of the phenolic metabolism and of the oxidative stress response was observed. At 80 days after grafting a higher expression of transcription factors regulating vascular maintenance, differentiation and proliferation was obtained in the most compatible combination. Moreover, lower expression levels of microRNAs potentially targeting important transcription factors related to plant development was observed in the more compatible combination when compared to the less compatible one. Conclusion In this context, a set of regulators was selected as potential expression markers for early prediction of a compatible grafting.