Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas
Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas
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DOI：
10.1186/s12935-018-0700-2
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发表时间：
2018-12-11
影响因子：
5.8
通讯作者：
Yuan, Aping
中科院分区：
文献类型：
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作者：
Cui, Guanglin;Ren, Jingli;Yuan, Aping
研究背景白细胞介素（IL）-33及其主要功能受体ST 2参与肿瘤的发生、发展过程。我们描述了人食管鳞状细胞癌（ESCC）中IL-33/ST 2轴在不同区室中的细胞和临床病理特征。结果免疫组化结果显示IL-33-IR和ST-2-IR在食管鳞癌细胞和肿瘤间质细胞中的表达增加，并与临床病理特征如TNM分期和淋巴结转移有关。然而，Kaplan-Meier分析显示，ESCC肿块和间质中IL-33阳性和ST 2阳性细胞的密度均与ESCC患者的总生存率无关。免疫荧光双染色细胞特征分析表明，这些IL-33阳性和ST 2阳性细胞在ESCC中具有较高的增殖率，IL-33-IR经常与ST 2-IR共同表达在ESCC和基质cells.ConclusionSignificant改变细胞特征的IL-33/ST 2轴在ESCC与先进的临床病理变量。提示IL-33/ST 2轴可能参与人食管鳞癌的发生发展。
BackgroundEmerging evidence has suggested that interleukin (IL)-33 and its primary functional receptor ST2 are involved in the pathogenesis of tumorigenesis.MethodsUsing immunohistochemistry (IHC) and double immunofluorescence staining, we characterized the cellular and clinicopathological features of the IL-33/ST2 axis in different compartments in human esophageal squamous cell carcinoma (ESCC) surgical specimens.ResultsIHC data revealed an increased expression of IL-33-immunoreactivity (IR) and ST2-IR located in both ESCC cells and tumor stromal cells; which were associated with advanced clinicopathological features such as TNM stages and node involvement. However, the Kaplan-Meier analysis showed that densities of neither IL-33 positive nor ST2 positive cells in both the ESCC mass and stroma were associated with the overall survival rate in patients with ESCC. Double immunofluorescence staining for cellular feature analysis demonstrated that these IL-33 positive and ST2 positive cells in ESCCs were with a high proliferation rate, and IL-33-IR was frequently co-expressed with ST2-IR in both ESCC and stromal cells.ConclusionSignificant altered cellular features of the IL-33/ST2 axis in ESCCs were associated with advanced clinicopathological variables. The data suggest that the IL-33/ST2 axis might be involved in the progression of human ESCCs.